Term
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Definition
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Term
| What happens after translation of the signal? |
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Definition
Bind SRP and this stops translation
Signal recognition particle |
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Term
| What happens after SRP gets bound |
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Definition
| Docks on SRP receptor on the ER membrane |
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Term
| Step after SRP receptor becomes bound on ER membrane? |
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Definition
| Translation restarts, lose SRP |
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Term
| What happens after you lose SRP and restart translation at the ER membrane |
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Definition
| A signal enters and you dissociate from SRP signal receptor |
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Term
| What happens after disassociation from SRP signal receptor? |
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Definition
| The signal gets cleaved, and ribosomal subunits are released and get recycled |
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Term
| What happens after the signal is cleaved? |
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Definition
N-glycosylation (adding a carbohydrate chain via dolachol)
N = asperagene |
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Term
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Definition
COP II Coating protein binds (ER -> Golgi)
Uses a V snare and T snare to transport (these need Rab protein and a tethering protein) |
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Term
| What happens if something gets transported to the golgi that shouldn't have? |
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Definition
| It binds to KDEL receptors and those bind to Cop I and bring it back to the ER |
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Term
| What happens in the cis golgi (to packages in the proper place) |
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Definition
| Phosphylation of mannose (prepares for golgi to lysosome) |
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Term
| Where does mannose go after it has been phosphorylated? |
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Definition
Binds to M6P Receptor in the trans golgi
The M6P receptor binds to clathrin on cytosolic side |
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Term
| Where does the package go after the trans golgi? |
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Definition
| Trans golgi to early endosome to late endosome to vesicle |
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Term
| Where are other options for the package to go? (aside from lysosome) |
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Definition
Regulated secretion - package aggregates into a secretory granule (stored until release)
Default constuitive - package goes straight to plasma membrane |
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Term
Membrane proteins are glysylated proteins
True or false |
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Definition
| True, membrane proteins are glycosylated proteins |
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Term
| What is needed to uncoat Clathrin? |
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Definition
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Term
What coat is used for endocytosis?
(as well as golgi to vesicle) |
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Definition
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Term
| How does clathrin bind in endocytosis? |
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Definition
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Term
| What is needed to completely bud of vesicle |
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Definition
| Dynamin + GTP hydrolysis for energy |
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Term
| What happens when LDL (made of apolipid protein and esters of cholesterol) gets endocytosed |
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Definition
Goes to a lysosome, LDL receptors get recycled
Gets broken down to free cholestrol in lysosome
Free cholesterol slows down smooth ER synthesis of cholesterol |
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Term
| Where can preproprotein be found? |
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Definition
| In the lumen, but signal likely to be cleaved right away |
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Term
| Where can proprotein be found? |
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Definition
| All the way through the golgi, but is cleaved upon arrival |
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Term
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Definition
No ribosomes
Site for budding off vesicles |
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Term
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Definition
Bound ribosomes (cytosolic side)
Integration of transmembrane proteins
Recieves proteins to be secreted
ER-Lumen specific proteins |
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Term
How are free and bound ribosomes different?
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Definition
| only in terms of the mRNA (messenger RNA) they are translating |
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Term
| Ribosomes translate mRNA bind to WHAT end, WHAT2 is made first |
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Definition
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Term
| mRNAs destined for ER have a signal sequence near the 5' end – first part to be translated then you end up with what? |
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Definition
a signal peptide
Signal sequence (peptide) at NH2 terminus of the polypeptide |
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Term
| Cotranslational Transport |
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Definition
1.SRP meets SRP receptor at ER membrane
2.Translation resumes
3.Release SRP
4.SRP receptor separates
5.Signal sequence enters translocator (pore)
6.As translation continues, polypeptide moves through channel |
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Term
| xTransport into ER lumen is occuring how |
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Definition
| Simultaneously with translation |
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Term
| When a polypeptide moves into the lumen and the signal sequence is cleaved off what happens? |
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Definition
| Ribosomal subunits are released and go back to being free ribosomes |
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Term
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Definition
| Helps pull the protein across the membrane |
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Term
| Bacteria can use post translational transport but... |
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Definition
there is no ER so they are pushing proteins across plasma membrane -> Secretions
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Term
SecA - ATPase (atp provides energy)
Destinations |
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Definition
ER lumen
ER membrane transmembrane proteins
NH2 end in cytosol
NH2 end in ER lumen
NH2 + COOH ends in cytosol or in lumen |
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Term
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Definition
Oligasaccharide is attached to a membrane lipid = dolichol (high energy phosphate bond, breaking of bond allows for it to be attached to protein
Transfer oligosaccharide to protien
Attachment is to an asparagine (amino acid) |
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Term
| Where is the glyco part of N-glycosylation |
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Definition
| in cytosol, never ER lumen |
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