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Bio344 Exam 3 Quick Review
N/A
36
Biology
Undergraduate 3
11/04/2010

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Cards

Term
First step in exocytosis
Definition
Translate the signal
Term
What happens after translation of the signal?
Definition

Bind SRP and this stops translation

 

Signal recognition particle

Term
What happens after SRP gets bound
Definition
Docks on SRP receptor on the ER membrane
Term
Step after SRP receptor becomes bound on ER membrane?
Definition
Translation restarts, lose SRP
Term
What happens after you lose SRP and restart translation at the ER membrane
Definition
A signal enters and you dissociate from SRP signal receptor
Term
What happens after disassociation from SRP signal receptor?
Definition
The signal gets cleaved, and ribosomal subunits are released and get recycled
Term
What happens after the signal is cleaved?
Definition

N-glycosylation (adding a carbohydrate chain via dolachol)

 

N = asperagene

Term
After N-glycosylation
Definition

COP II Coating protein binds (ER -> Golgi)

 

Uses a V snare and T snare to transport (these need Rab protein and a tethering protein)

Term
What happens if something gets transported to the golgi that shouldn't have?
Definition
It binds to KDEL receptors and those bind to Cop I and bring it back to the ER
Term
What happens in the cis golgi (to packages in the proper place)
Definition
Phosphylation of mannose (prepares for golgi to lysosome)
Term
Where does mannose go after it has been phosphorylated?
Definition

Binds to M6P Receptor in the trans golgi

 

The M6P receptor binds to clathrin on cytosolic side

Term
Where does the package go after the trans golgi?
Definition
Trans golgi to early endosome to late endosome to vesicle
Term
Where are other options for the package to go? (aside from lysosome)
Definition

Regulated secretion - package aggregates into a secretory granule (stored until release)

 

Default constuitive - package goes straight to plasma membrane

Term

Membrane proteins are glysylated proteins

 

True or false

Definition
True, membrane proteins are glycosylated proteins
Term
What is needed to uncoat Clathrin?
Definition
ATP
Term

What coat is used for endocytosis?

 

(as well as golgi to vesicle)

Definition
Clathrin
Term
How does clathrin bind in endocytosis?
Definition
Via adaptin
Term
What is needed to completely bud of vesicle
Definition
Dynamin + GTP hydrolysis for energy
Term
What happens when LDL (made of apolipid protein and esters of cholesterol) gets endocytosed
Definition

Goes to a lysosome, LDL receptors get recycled

 

Gets broken down to free cholestrol in lysosome

 

Free cholesterol slows down smooth ER synthesis of cholesterol

Term
Where can preproprotein be found?
Definition
In the lumen, but signal likely to be cleaved right away
Term
Where can proprotein be found?
Definition
All the way through the golgi, but is cleaved upon arrival
Term
Smooth ER
Definition

No ribosomes

Site for budding off vesicles

Term
Rough ER
Definition

Bound ribosomes (cytosolic side)

Integration of transmembrane proteins

Recieves proteins to be secreted

ER-Lumen specific proteins

Term

How are free and bound ribosomes different?

 

 

Definition
only in terms of the mRNA (messenger RNA) they are translating
Term
Ribosomes translate mRNA bind to WHAT end, WHAT2 is made first
Definition

5'

 

END TERMINUS

Term
mRNAs destined for ER have a signal sequence near the 5' end – first part to be translated then you end up with what?
Definition

a signal peptide

 

Signal sequence (peptide) at NH2 terminus of the polypeptide

Term
Cotranslational Transport
Definition
1.SRP meets SRP receptor at ER membrane
2.Translation resumes
3.Release SRP
4.SRP receptor separates
5.Signal sequence enters translocator (pore)
6.As translation continues, polypeptide moves through channel
Term
xTransport into ER lumen is occuring how
Definition
Simultaneously with translation
Term
When a polypeptide moves into the lumen and the signal sequence is cleaved off what happens?
Definition
Ribosomal subunits are released and go back to being free ribosomes
Term
in Lumen, BiP does what
Definition
Helps pull the protein across the membrane
Term
Bacteria can use post translational transport but...
Definition

there is no ER so they are pushing proteins across plasma membrane -> Secretions

 

 

Term

SecA - ATPase (atp provides energy)

Destinations

Definition

ER lumen

ER membrane transmembrane proteins

NH2 end in cytosol

NH2 end in ER lumen
                        NH2 + COOH ends in cytosol or in lumen

Term
N-glycosylation
Definition
Oligasaccharide is attached to a membrane lipid = dolichol (high energy phosphate bond, breaking of bond allows for it to be attached to protein
Transfer oligosaccharide to protien
Attachment is to an asparagine (amino acid)
Term
Where is the glyco part of N-glycosylation
Definition
in cytosol, never ER lumen
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