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BIO205 lab practical
lab 1-13
100
Microbiology
Undergraduate 2
10/18/2009

Additional Microbiology Flashcards

 


 

Cards

Term
[image]
Definition

enterobius vermicularis ova

route of transmission: infected people, towels, tubs

Term
[image]
Definition

ascaris lumbricoides ova

route of transmission: fecal-oral route

Term
[image]
Definition

Necator americanus ova

route of transmission: infected larvae penetrate intact skin of feet when in contact with soil

Term
[image]
Definition

trichinella spiralis larvae

route of transmission: consumption of raw or insufficiently cooked pork or bear meat.

Term
[image]
Definition

clonorchis sinensis ova

route of transmission:

feces->snail->fish->human

Term
[image]
Definition

schistosoma mansoni ova

route of transmission:

feces->miracidia->snail->cercaria->human skin

Term
[image]
Definition

Taenia species proglottid

route of transmission: undercooked beef or pork

Term
[image]
Definition

taenia species ova

route of transmission: raw/undercooked pork of beef

Term
[image]
Definition

entamoeba histolytica trophozoite

route of transmission: cyst ingested from contaminated food or H2O

Term
[image]
Definition

entamoeba histolytica cysts

route of transmission: cysts ingested in contaminated food or H2O

Term
[image]
Definition

trichomonas vaginalis trophozoites

route of transmission: intimate contact

Term
[image]
Definition

toxoplasma gondii trophozoites

route of transmission: cat feces or undercooked meat

Term
[image]
Definition

cryptosporidium oocyst

route of transmission: animal fecal contamination

Term
[image]
Definition

giardia lamblia trophozoites

route of transmission: unpurified H2O

Term
[image]
Definition

giardia lamblia cyst

route of transmission: unpurified H2O

Term
[image]
Definition
balantidium coli trophozoites route of transmission: lack of good hygeine and crowding
Term
[image]
Definition
balantidium coli cyst route of transmission: lack of good hygeine and crowding
Term
[image]
Definition

trypanosoma trophozoites

route of transmission: bug bites

Term
[image]
Definition

plasmodium trophozoites

route of transmission: mosquito bites

Term
Explain the purpose of the immersion oil when using the 100x objective
Definition
to avoid light refraction
Term
State the route of transmission for Enterobius vermicularis ova (pinworms)
Definition
typically is from person to person via the fecal-oral route
Term
State the route of transmission for Ascaris lumbricoides fertilized ova (roundworm)
Definition
fecal-oral route
Term
state the route of transmission for Necator americanus ova (hookworm)
Definition
barefoot contact with soil
Term
State the route of transmission for Trichinella spiralis larvae
Definition
eating bear or pig meat
Term
state the route of transmission for Clonorchis sinensis ova
Definition
feces->snail->fish->skin
Term
state the route of transmission for Schistosoma mansoni ova
Definition
feces->snail->skin
Term
state the route of transmission of Taenia saginata or solium
Definition
eating raw or undercooked beefor pork
Term
Define cysticercosis
Definition
Cysticercosis is a systemic illness caused by dissemination of the larval form of the pork tapeworm, Taenia solium
Term
define ova
Definition
egg cell
Term
define scolex
Definition
The knoblike anterior end of a tapeworm, having suckers or hooklike parts that in the adult stage serve as organs of
Term
define proglottid
Definition
body segments of worm that extend from the head
Term
define monoecious
Definition
having male and female reproductive organs in the same plant or animal
Term
define dioecious
Definition
having male and female reproductive organs in separate plants or animals
Term
state the route of transmission for entamoeba histolytica
Definition
cysts ingested in contaminated food or H2O
Term
state the route of transmission for trichomonas vaginalis
Definition
intimate contact
Term
state the route of transmission for toxoplasma gondii
Definition
cat feces or undercooked meat
Term
state the route of transmission for cryptosporidium
Definition
animal fecal contamination
Term
state the route of transmission for giardia lamblia
Definition
unpurified H2O
Term
state the route of transmission for balantidium coli
Definition
lack of good hygeine and crowded areas
Term
state the route of transmission for trypanosoma
Definition
bug bites
Term
state the route of transmission for plasmodium
Definition
mosquito bites
Term
Name the disease caused by Entamoeba histolytica
Definition
amoebic dysentry
Term
Name the disease caused by Trypanosoma brucei
Definition
African sleeping sickness
Term
Name the disease caused by Trypanosoma cruzi
Definition
Changas disease
Term
Name the disease caused by Plasmodium
Definition
malaria
Term
Define ubiquitous
Definition
everywhere
Term
Define pure culture
Definition
Isolation of a single species of microbe 
Term
Define colony
Definition
cells that descend from a single parent cell that reproduced asexually
Term
Define turbidity
Definition
Cloudiness
Term
Define media
Definition
Nutrient material suitable for cultivation of microorganisms
Term
Define inoculum
Definition
Sampling of a bacterial culture
Term
Define aseptic technique
Definition
Aseptic technique refers to a procedure that is performed under sterile conditions
Term
Explain the importance of allowing the loop to cool before obtaining your bacterial sample
Definition
If it is hot it could kill the bacteria
Term
correct procedure to streak for isolation
Definition
[image]
Term
explain why the agar plates are labeled on the bottom and inverted when incubated
Definition
The condensation that forms inside the dish can cause streaking through the growth on the plates. Thus, causing poor or invalid test results.
Term
state the criteria used to describe a bacterial colony
Definition
shape, margin, elevation, texture, size, appearance, pigmentation, and optical property
Term
list, in order, the reagents used un the traditional gram stain procedure
Definition
Cyrstal violet
Gram's iodine
Acetone-alcohol
Safranin
Term
State the function of crystal violet stain used in the Gram stain
Definition
Primary stain
Term
State the function of Iodine stain used in the Gram stain
Definition
mordant that combines with crystal violet in the cell
Term
State the function of acetone or ethanol used in the Gram stain
Definition
the decolorizer
Term
State the function of safranin used in the Gram stain
Definition
counterstain or secondary stain
Term
explain the mechanism of the gram stain (why gram negative cells lose the primary dye during decolorization)
Definition
It is a differential staining method
of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based
on the chemical and physical properties of their cell walls.
Term
Explain the difference between a "simple" stain and a "differential" stain.
Definition
simple stain bacteria stain one color.
differential stain two dyes are used and cause certain bacteria types to stain contrasting colors depending on their properties and characteristics.
Term
State two organisms that are identified using the acid-fast stain
Definition
mycobacterium annd nocardia
Term
[image]
Definition
acid-fast are pink. non acid-fast are purple
Term
explain why certain organisms are "acid-fast"
Definition
they contain large amounts of lipid called mycolic acid, which makes them resistant to staining and disinfectants.
Term
What is the decolorizer in acid fast stain?
Definition
Acid/Alcohol
Term
What is the decolorizer in gram stain?
Definition
Acetone/Ethanol
Term
state, in order, the three reagents used in the acid-fast stain
Definition
1) Carbolfuchsin
2) Acid-alcohol
3) Methylene blue
Term
Name two medically important genera of bacteria that produce endospores
Definition
Bacillus and Clostridium
Term
[image]
Definition
identify the vegetative cells and the spores
Term
what color does a vegetative cell stain in the schaeffer-fulton stain?
Definition
red
Term
what color does an endospore stain in the schaeffer-fulton stain?
Definition
green
Term
[image]
Definition
enterobius vermicularis ova
pinworm
spread person to person
fecal-oral
Term
[image]
Definition
ascaris lumbricoides ova
roundworm
spread through fecal-oral route
Term
[image]
Definition
necator americanus ova
hookworm
route of transmission walking barefoot through soil
Term
[image]
Definition
trichinella sipralis larvae
can become infected from eating undercooked meat.
Term
[image]
Definition
clonorchis sinensis ova
route of transmission: eating raw or undercooked freshwater fish
Term
[image]
Definition
schistosoma mansoni ova
route of transmission: skin contact with infected water
Term
[image]
Definition
taenia species proglottid
route of transmission: by digestion of undercooked pork or beef
Term
[image]
Definition
taenia species ova
route of transmission: by digestion of undercooked pork or beef
Term
[image]
Definition
entamoeba histolytica trophozoite
route of transmission: =ingesting cysts in contaminated food or water
Term
[image]
Definition
entamoeba histolytica cysts
route of transmission: ingesting cysts in contaminated food or water.
Term
[image]
Definition
trichomonas vaginalis trophozoites
transmitted by initmate contact between individuals
Term
[image]
Definition
toxoplasma gondii trophozoites
route of transmission: cat feces or undercooked meat of an infected animal
Term
[image]
Definition
cryptosporidum oocyst
route of transmission: from the feces of an infected animal to the mouth of another
Term
[image]
Definition
giardia lamblia trophozoites
route of transmission: drinking unpurified water
Term
[image]
Definition
giardia lamblia cyst
drinking unpurified water
Term
[image]
Definition
balantidium coli trophozoites
common in prisons and nursing homes where there is a lack of good hygeine
Term
[image]
Definition
balantidium coli cyst
common in prisons and nursing homes where there is a lack of good hygeine
Term
[image]
Definition
trypanosoma trophozoites
route of transmission: infection by a bug bite
Term
[image]
Definition
plasmodium trophozoites
infection occurs through bugbite
Term
[image]
Definition
causes dysentry
Term
[image]
Definition
causes malaria
Term
what disease does trypanosoma cruzi cause?
Definition
changas disease
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