Shared Flashcard Set

Details

Bio Sci 194S
5 Hughes Videos
14
Biology
Undergraduate 3
06/09/2009

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Cards

Term
1) substitution with less toxic chemicals
2) smallest amount of material
Definition
In the video about assessing toxic risks, what 2 guides should you use when planning a new experiment:
Term
the dose, duration, frequency, and route of exposure
Definition
The extent of exposure to a toxic compound is determined by:
Term
Acute toxicity
Definition
LD50 measures acute or chronic toxicity?
Term
Tells co-workers, put up sign saying "EMS in use", tell co-workers to ewar gloves when reaching into hood.
Definition
When the very toxic compound EMS was in use in the hood, and the researcher left, what did she do do?
Term
speed; tube
Definition
Centrifugation hazards: rotors are designed to run at a maximum ______ with loads at a specific weight. It is also critical to make sure you have a ____ that fits correctly in the rotor.
Term
aersol; biological safety cabinet
Definition
When centrifuging, first decide what level containment is needed. When centrifuging infectious agents always 1) use _________ containment tubes and rotors and 2) load and unload in a ____________ .
Term
10 minutes
Definition
When working with infectious agents, wait ____ minutes after the centrifuge rotor stops before opening door and if there's a spill, close the door and plan your cleanup.
Term
salts; de-ionized water; logs
Definition
Corrosion or metal fatigue can cause rotor failure so rotors must be maintained and retired properly. Important steps to prevent rotor failure include keeping rotors clean and dry, wash immediately if spills occur or if ____ or corrosives are used, rinse with __________, inspect for pitting, color change and ALWAYS maintain the rotor _____ on an ultraspeed rotor.
Term
corrosive; burns
Definition
In the Chemical hazards video, they demonstrated a person equilibrating phenol: This chemical is ___________ and causes serious _________ on contact with skin.
Term
penetrate the skin; inhalation
Definition
Adding chloroform enhances phenol's abilty to ____________ ________. Chloroform also causes cancer, organ damage, and is toxic by _________ and skin absorption
Term
fume hood; splash
Definition
When extracting proteins from DNA with phenol/chloroform use a ________ _________!! Empty in appropriate waste container, DON'T ______!
Term
tritium (3H)
Definition
The beta emitter ____ can not be detected by a GM counter and it is hazardous by ingestion.
Term
aerosol; screw; container
Definition
To prevent radioactive contamination, you should use: _________ resistant pipet tips _________ top eppendorf tubes for heating and centrifuging Regularly check gloves, equipment and centrifuge Double glove in case you contaminate your hand Use a secondary ___________ in water bath
Term
prior; aerosol; bleach; alcohol; biosafety level 2
Definition
When performing tissue culture taking these steps will decrease your exposure to potentially infectious agents like HIV and HPV: make sure everything is in the hood ________ to beginning use __________ proof pipets disposable pipets are placed in a container with ________ solution for decontamination the hood is decontaminated with ____________, then 0.5% iodine solution When working with human cells and tissues use _________ practices and techniques, avoid aerosols or splashes and the vaccuum trap must contain __________.
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