Term
| Why are restriction enzymes used? |
|
Definition
| Used to cut and degrade viruses that enter their cells. |
|
|
Term
| Why is bacteria a good choice for transformation? |
|
Definition
| Single celled, use plasmid DNA, and reproduce quickly |
|
|
Term
|
Definition
1.)CaCl₂treatment (TS)-positive charge of CA₂+ neutralizes negative charge of DNA and cell membrane
2.)Incubation on ice-slows cell membranes
3.)Heat-shock-increases permeability of cell membrane
(2 and 3 allow plasmid to be taken in by bacteria cells)
4.)Nutrient broth incubation-allows beta lactamase gene to be expressed (for antibiotic resistance) |
|
|
Term
| What genes are contained in the pGLO plasmid? |
|
Definition
| Beta lactamase, green fluorescent protein, and araC regulator protein |
|
|
Term
|
Definition
| Produces carbon dioxide and alcohol; causes bread dough to rise |
|
|
Term
|
Definition
| Produces lactic acid; produced in muscles when body can't supply enough oxygen to the tissues |
|
|
Term
|
Definition
6CO₂+ 6H₂0 ----> C₆H₁₂ O₆ + 6O₂
(Carbon dioxide + water --light--> sugars + oxygen) |
|
|
Term
| Cell Respiration Equation |
|
Definition
60₂+ C₆H₁₂O₆---> 6CO₂+6H₂O + Energy
(Oxygen + glucose---> carbon dioxide + water + energy) |
|
|
Term
| What is alternation of generations? |
|
Definition
| Process in which many algae switch back and forth between haploid and diploid stages of their life cycles. |
|
|
Term
| What to plants need to survive on land? |
|
Definition
| Sunlight, water, minerals, gas exchange, and the transport of water and nutrients throughout the plant body. |
|
|
Term
|
Definition
| Solutions of nutrients and carbohydrates produced by photosynthesis |
|
|
Term
|
Definition
| Water upward from the roots to every part of a plant. |
|
|
Term
|
Definition
| Porelike openings in the underside of the leaf that allow carbon dioxide and oxygen to diffuse into and out of the leaf. |
|
|
