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Bio Exam1
February 17, 2010
51
Biology
Undergraduate 1
02/11/2010

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Term
Amino acids is composed of 5 things:
Definition
An alpha carbon
A hydrogen
An amino group
A carboxyl group
A R group
Term
Every 3-4 residues the chain can change from...
Definition
hydrophobic-hydrophilic.
Term
Good and bad a helix formers
Definition
Good a helix formers: Ala, Glu, Leu, and Met.

Poor a helix formers: Pro, Gly, Tyr, and Ser.
Term
Two types of proteins are based on their tertiary structure
Definition
Fibrous
globular
Term
For protein folding and stability, it's important to have...
Definition
Weak non-covalent interactions and one covalent bond
Term
Quaternary Structure is made of...
(plus give an example)
Definition
Made of multiple sub-units
(Weak chemical interactions between subunits)


Example: hemoglobin
Term
conformation of protein
Definition
three dimensional structure of proteins
Term
native conformation
Definition
proteins that have the correct three dimensional structure
Term
Function of Carbohydrates
Definition
Energy storage
Structural component
Term
Structure of Carbohydrate
Definition
Monomers (1 sub-unit)
2X H atoms/1X C and 1X O2
Polymers (multi-subunit)
Term
Examples of Carbohydrates
Definition
Glucose (monomer)

Starch (polymer): energy storage made in plant cells

Cellulose (polymer): structural component of bark, leaves, etc.

Glycogen (polymer): energy storage made by muscle cells
Term
Three types of Lipids
Definition
Fats
Oils
steroids
Term
Function of lipids
Definition
Form membranes (cell and organelle)
Energy storage

Warmth

Cushioning

Used to make vitamins and steroid hormones
Term
Structure of lipids
Definition
Polymers (the hydrophobic section lacks oxygen)

Ring structure (steroids)
Term
Examples of lipids
Definition
Palmitic acid, cholesterol
Term
Membrane functions
Definition
Selective barrier

Provides the formation of compartments

Localization of enzymatic reactions

Cell-cell communication

Transmission/reception of signals
Term
Membrane phospholipids are synthesized in...
Definition
...cytosolic side of the smooth ER.
Term
Flippases are used to...
Definition
...move the phospholipids to the other side of the membrane.
Term
Extracellular domain of membrane proteins is
Definition
glycosylated
Term
Fluidity is important because it allows...
Definition
...selective movement of molecules across the membrane.
Term
Fluidity depends on:
Definition
Length of fatty acid chains (16-18 C most common)

The proportion of saturated and unsaturated fatty acids

Number of cholesterol molecules in the membrane (only in animal cells)
Term
Movement of lipids in a cell membrane was studied using
Definition
fluorescence recovery experiments
Term
Uniporters
Definition
one type of molecule is transported
Term
Cotransporters
Definition
two types of molecules are transported at the same time
Term
Hypertonic solution
Definition
Net flow of water out of cell; cell shrinks
Term
Hypotonic solution
Definition
Net flow of water into cell; cell swells or even bursts
Term
channel or carrier protein (transporter) is used to
Definition
move molecules through membrane
Term
energy used for the movement of molecules across membrane comes from...
Definition
concentration gradient
Term
aquaporin
Definition
When water is transported by a transmembrane protein
Term
When ions build up on one side of a plasma membrane...
Definition
they establish a concentration gradient and an electrochemical gradient.
Term
Molecules and ions always diffuse through
Definition
channels down their electrochemical gradients, which is called passive transport.
Term
Passive transport requires...
Definition
no energy and decreases the charge and concentration differences between the cell's exterior and interior
Term
Facilitated diffusion can occur through
Definition
channels or carrier proteins/transporters, which change shape during the transport process
Term
Facilitated diffusion by transporters occurs only down an
Definition
electrochemical gradient, reducing differences between solutions
Term
Glucose is a building block for
Definition
important macromolecules and a major energy source, but lipid bilayers are only moderately permeable to glucose.
Term
What increases membrane permeability to glucose?
Definition
A glucose transporter named GLUT-1
Term
Three main things that happen during ATP
Definition
Molecules move from [low] to [high]

Proteins are needed to be carriers

Ions are transported actively
Term
Functions of ATP
Definition
Allows the maximum number of nutrients to be taken by the cell.

Allows the maximum removal of cellular waste products

Allows cells to communicate (ex: action potential)
Term
How the Sodium Potassium Pump works:
Definition
1. Three binding sites within the protein have a high affinity for sodium ions.

2. Three sodium ions from the inside of the cell bind to these sites.

3. A phosphate group from ATP binds to the protein. In response, the protein changes shape.

4. The sodium ions leave the protein and diffuse to the exterior of the cell.

5. In this conformation, the protein has binding sites with a high affinity for potassium ions.

6. Two potassium ions bind to the pump.

7. The phosphate group drops off the protein. In response, the protein changes back to its original shape.

8. The potassium ions leave the protein and diffuse to the interior of the cell. These 8 steps repeat.
Term
Characteristics of all cells (living things)
Definition
Have semi-permeable membranes

Can receive stimuli and respond to it.

Can process organic components to harvest energy.

Composed of the same organic and inorganic compounds.

Can reproduce

Use DNA to store genetic information.

The information is used to make RNA to make protein.
Term
Characteristics of Prokaryote Cells
Definition

Very small (20 mm)

Single celled-organisms
Cell membrane and cell wall
No organelles
All biochemical reactions occur in the cytoplasm or cell membrane.
One circular chromosome
Some of them have flagella
No (actin, microtubule-based) cytoskeleton
Term
Characteristics of Eukaryote Cells
Definition

 

Single or multi-cellular organisms.
All have cell membranes, but some have cell walls (plants).
Organelles
Cellular compartments most biochemical reactions take place.
A nucleus
Containing many chromosomes
Cytoskeleton
Some have flagella for movement.
Protozoans, plants, animals, fungi
Term

 

The compartmentalization of eukaryotic cells increases chemical reaction efficiency by ...
Definition

...

separating incompatible chemical reactions and grouping enzymes and substrates together.
Term
The nucleolus is where what occurs?
Definition

 

Ribosomal RNA synthesis occurs
Term
Function of Golgi Apparatus
Definition

 

Protein processing (glycosolation)
It receives products from the rough ER and sends finished products to the cell surface in vesicles.
Term
Function of Smooth ER
Definition

 

-contains enzymes for synthesizing
-fatty acid and lipid synthesis.
Term

 

Peroxisomes are
Definition

 

globular organelles bound by a single membrane in which oxidation reactions occur. Also processes fatty acids.
Term

 

Lysosomes are
Definition

 

single-membrane-bound centers for storage and/or waste processing. Materials are delivered to the lysosomes by three processes: phagocytosis, autophagy, and receptor-mediated endocytosis.
Term

 

Endocytosis
Definition

 

process by which the cell membrane can pinch off a vesicle to bring outside material into the cell
Term

a) Thylakoids are stacked into piles called:

b) The solution outside of the thylakoid is called:

Definition

a) grana

b) stroma

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