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BIO 230 Microbiology XI
DNA Replication and Protein Synthesis in Bacteria
36
Biology
Undergraduate 2
07/31/2009

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Term

Nitrogenous Base

Sugar molecule

Phosphate Group

Definition
DNA contains these three molecules
Term
4
Definition
Number of different nucleotides
Term
dinucleotide
Definition
2 nucleotides bonded together
Term
polynucleotide
Definition
3 or more nucleotides bonded together
Term
hydrogen bond
Definition
type of bond between DNA strands
Term

purines

pyridamines

Definition
two categories of nitrogenous bases
Term

Adenine

Guanine

Definition
Purines
Term

Cytosene

Thymine

Uracil

Definition
pyridamines
Term

DNA                                     RNA

Deoxyribose                         Ribose

Thymine                               Uracil

Double stranded                  Single Stranded

 

Definition
Three differences between DNA and RNA
Term
polynucleotide
Definition
DNA is a _____________ (polynucleotide/dinucleotide).
Term
DNAse
Definition
Enzyme that cuts the circular DNA into a linear strand.
Term

1. Circular DNA is cut

2. Cut ends are attached to 2 sites on the cell membrane

3. DNA strands unwind

4. New DNA nucleotides attach to old bases

5. New phosphate sugar backbone forms

6. 2 new strands rewind

7. 2 double strands separate from cell membrane

8. DNA strands reform ino circle using ligase

9. Strands separate to each side of the cell


Definition
Steps of DNA replication
Term
Transcription
Definition
copy of DNA code for 1 protein is made by mRNA
Term
Translation
Definition
tRNA carries a specific amino acid to ribosome where amino acids are assembled to make a protein as determined by mRNA
Term
T A C
Definition
DNA start codon
Term
A C T
Definition
DNA stop codon
Term
codons
Definition
3 mRNA bases that code for amino acids
Term
AUG
Definition
start codon in RNA
Term
methianine
Definition
What AUG codes for...
Term
UGA, UAA, UAG
Definition
Stop codons for RNA
Term
64
Definition
There are a total of _______ codons.
Term
anticodon
Definition
Codon on bottom of tRNA that links to the mRNA when making amino acids.
Term
mutation
Definition
change in DNA bases of a gene
Term

substitution

deletion

addition

Definition
three types of DNA mutations
Term

1:106

( 1 in a million)

Definition
Number of bacteria that mutate spontaneously
Term

1:103

(1 in 1,000)

Definition
Number of bacteria that can be induced to mutate
Term

radiation

chemicals

Definition
Two agents that will induce mutation in baceria.
Term

Tay Sachs Disease

Sickle Cell Anemia

Definition
Two examples of diseases caused by single base substitutions.
Term
mRNA
Definition
Copies the genetic information in DNA by complementary base pairing acting as a "message" between the DNA of the nuclear body and the ribosomes of the cytoplasm where protein synthesis occurs.
Term
tRNA
Definition
Becomes charged with a specific amino acid, carries the amino acid to the ribosome and inserts the correct amino acid in the proper place according to the genetic message.
Term
makes mRNA ( a copy of the gene)
Definition
What RNA polymerase does...
Term

Double stranded DNA opens up allowing RNA polymerase to copy it.

 

This copy is called mRNA.

 

When the copy is complete, the two strands of DNA are closed up again.

Definition
How transcription occurs...
Term

mRNA binds to the ribosome at the start codon (AUG) where tRNA binds to make methianine

 

Definition
What happens at Initiation...
Term

tRNA binds to each subsequent codon on the mRNA-ribosome complex

 

Each new amino acid is added to the bottom of a growing stack/chain of amino acids.

 

 Once an amino acid is made, the uncharged tRNA leaves the ribosome and goes to find another amino acid until the mRNA codes for it.

 

Definition
How elongation works
Term
When the ribosome shifts to a nonsense/stop codon on the mRNA, the chain of amino acids (polypeptide chain) is released. This is now a finished protein.
Definition
What happens at Termination
Term

Initiation

Elongation

Termination

Definition
Three steps of translation...
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