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| DNA polymerase III can _______ |
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| nucleotide excision repair |
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Definition
(1) enzymes detect an irregularity and cut damaged strand (2) enzyme excises nucleotides on damaged strand (e) DNA polymerase fills in gaps in 5-3 direction (4) DNA ligase links it together |
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• binds at replication fork • breaks H-bonds between bases and opens double helix |
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• binds ahead of the replication fork • breaks covalent bonds in DNA backbone to relive twisting forces cause by opening the helix |
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| single-strand binding protein |
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Definition
• binds after replication fork • prevents H-bonds between bases and stabilize single-stranded DNA |
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only one primer needed polymerase moves toward replication fork made continuously |
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polymerase moves away from replication fork made in segments multiple primers needed |
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| both leading and lagging strand |
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Definition
| synthesized in 5-3 direction |
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| catalyzes synthesis of RNA primer |
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| DNA polymerase III's role in DNA synthesis |
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| holds DNA polymerase in place during strand extension |
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| removes RNA primer and replaces it with DNA |
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| catalyzes joining of Okazaki fragments into a continuous strand |
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