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BIO 111 - Midterm 2
genetics, inheritance, DNA, meiosis, mitosis
48
Biology
Undergraduate 1
02/21/2011

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Term
genotype
Definition
all the genes an organism carries
Term
phenotype
Definition
physical manifestations of the instructions (physical appearance of the individual)
Term
transcription
Definition
copy of gene's base sequence is made
Term
Where does transcription take place?
Definition
nucleus
Term
What is the first step of transcription?
Definition
Recognize & bind - RNA polymerase binds to one strand at promoter site
Term
What is the second step of transcription?
Definition
RNA Polymerase builds copy of gene mRNA
Term
What is the third step of transcription?
Definition
Terminate - RNA Polymerase find stop codon and releases mRNA
Term
What is the fourth step of transcription?
Definition
Capping & Editing: mRNA is processed - cap, tail, introns removed
Term
translation
Definition
copy from transcription is used to direct production of a protein
Term
Where does translation take place?
Definition
Cytoplasm
Term
What ingredients are needed for transcription?
Definition
free amino acids, mRNA, tRNA
Term
codon
Definition
3 bases read at a time
Term
star codon
Definition
AUG - methionine
Term
stop codon
Definition
UAA, UAG, UGA
Term
wobble base?
Definition
mistakes can be overlooked an still read as an amino acid with translation
Term
What are the 3 steps of translation?
Definition
1. Recognize and Initiate
2. Elongate
3. Terminate
Term
what is the first step of translation?
Definition
Recognize and Initiate - start sequence of mRNA recognized by tRNA. Binds to ribosomal subunits
Term
what is the second step of translation
Definition
Elongate - as ribosome moves along mRNA, tRNA with amino acid binds to next 3 bases on mRNA. tRNA released
Term
what is the third step of translation
Definition
Terminate - stop codon reached. Protein and mRNA releases from ribosome
Term
can the same mRNA be translated more than once before degrading?
Definition
yes - many molecules can be produced from one mRNA
Term
gamete
Definition
sex cell
Term
somatic
Definition
body cells
Term
What types of cells pass on information to the next generation?
Definition
sex cells (gametes)
Term
What are the different types of point mutations?
Definition
substitution, insertion, deletion
Term
What is a chromosomal change mutation?
Definition
larger scale - chunks of NDA are changed
Term
What are two ways the mutations are induced?
Definition
radiation and chemical
Term
Do all mutations cause change in proteins?
Definition
No - multiple codon/amino acids - introns aren't copied
Term
repressor
Definition
block RNA polymerase from binding DNA - if something is messed up it cannot copy it
Term
cell differentiation
Definition
each cell has all the DNA but only some is expressed
Term
what are the 5 steps of genetic engineering?
Definition
1. chop
2. amplify
3. insert
4. grow
5. identify
Term
bacteriophage
Definition
viruses that invade bacteria
Term
restriction enzymes
Definition
restrict foreign DNA from invading bacteria
Term
How are restriction enzymes used in genetic engineering?
Definition
they create start and stop signals so that chopping and be done correctly
Term
what are the three steps of polymerase chain reaction?
Definition
1. denaturation
2. annealing
3. extension
Term
denaturation phase of Polymerase Chain Reaction
Definition
heat added, double strand DNA splits creating two single strands
Term
annealing phase of Polymerase Chain Reaction
Definition
primer pairs up with template, copies polymerase to template and primer
Term
extension phase of Polymerase Chain Reaction
Definition
DNA building blocks complementary to template couple with primer -> makes double strand DNA
Term
cloning vector
Definition
plasmids that carry things in them (genetic engineering)
Term
why are bacteria good choices for insertion with genetic engineering?
Definition
bacteria has an accessory circle in the DNA (plasmids)
Term
stem cell
Definition
cells that have the ability to develop into any type of cell in the body
Term
biggest issue with Genetically modified organisms?
Definition
not enough genetic diversity
Term
genome
Definition
an organisms complete set of DNA
Term
chromosome
Definition
one or more unique places of DNA (tons of base pairs)
Term
gene
Definition
specific sequence of DNA
Term
does having more chromosomes make you a more intelligent/complex organism?
Definition
no
Term
allele
Definition
different version of the same trait (color of a flower)
Term
do prokaryotes have introns?
Definition
no
Term
how do prokaryotic cells divide?
Definition
binary fission
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