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Definition
Recognizes a specific sequence of bases anywhere within the genome and severs two covalent bonds
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Definition
| Fragment of DNA created by the restriction enzymes |
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Definition
| Act of cutting with restriction enzymes to make restriction fragments |
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Definition
| Enzymes make cuts in DNA to generate fragments with single-stranded ends that are protruding |
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Definition
Distinguishes DNA according to size - movement of a charge molecule through an electric field
-DNA moves down the gel towards a positive charge |
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Definition
| Specialized DNA sequence that can enter a living cell and signal its presence to an investigator by confering a detectable property on the host cell and provide means of replication for itself and the foreign DNA inserted into it |
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Definition
| A type of vector that is a circular strand of DNA |
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Definition
Antiobiotic resistance genes and other vector genes that make it possible to pick out cells harboring a particular DNA molecule
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Term
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Definition
| Put the antibiotic on the plate and test which cells are resistant |
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Definition
| Using beta-galactoidase to see if the lac Z gene is interupted or not, if it is not, the cells turn blue-- this means it did not take the insert |
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Definition
| A long-lived collection of cellular clones that contains copies of every single sequence in the whole genome intserted into a suitable vector |
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Definition
A library that on contains exon information obtained from RNA transcripts
-to produce them invitro viruses are used |
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Definition
| Purified fragments of single stranded DNA that are labeled with a radio active isotope |
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Definition
| Hybridization technique- restriction fragments from a complex genome are separated by gel electrophoresis and transfered by blotting |
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Term
| PCR Polymerase Chain Reaction |
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Definition
Used to isolate and make large quantities of a defined DNA fragment from a complex genome
Amplification is exponential
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Term
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Definition
| Mutant alleles that act dominantly |
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Term
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Definition
| Recessive alleles of genes whose normal alleles help put cell division on hold whether in terminally differenciated cells or cells with DNA damage |
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Term
| Loss of Function Mutation |
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Definition
They distrupt the gene function by altering the amino acid sequence of the protein product or by interfering with any step of gene expression
-Proteins with diminished or no biochemical acitivity |
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Term
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Definition
L-F
Mutations that remove all function
-Recessive to wild-type |
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Term
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Definition
| Take a cloned gene, use recombinant DNA technology to destroy function, and replace it with the wild-type gene in the genome with an activated cloned copy |
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Term
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Definition
Partial loss of function
-Can be beneficial in some cases |
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Term
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Definition
Loss of function under special circumstances
Ex: temp sensative |
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Term
| Dominant Negative Mutations |
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Definition
One good copy of an allele is not sufficient for normal development
-Haploinsufficiency |
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Term
| Gain of Function Mutation |
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Definition
Mutations that produce too much protein
-Usually dominant to the wild-type |
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Term
5% recombinance
30% recombinance
50% recombinance |
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Definition
- 5 linked and pretty close
-30 linked and further apart
-50 linked and far apart or not linked |
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Term
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Definition
"Knock out" mice
-replace one good copy of a gene w/ a mutant copy relies on homologous recombination |
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