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BCMB 3100 Test 1
Fun facts
4
Biochemistry
Undergraduate 3
06/24/2012

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Term
Chymotrypsin
Definition
  • digestive enzyme
  • serine protease (catalytic triad(SER195, HIS97, ASP102); differ in specitificity pockets 
  • specificity in large hydrophobic side chains (F,Y,W)
  • intermediate from rapid flow kinetics is acyl enzyme intermediate
  • all four modes of catalysis 
Term
Modes of Catalysis 
Definition
  • 2 types
  • chemical: Acid-Base and Covalent 
  • Binding: Proximity effect and Transition state stabilization
Term
Four levels of Pro. structure
Definition

primary, secondary, tertiary, and quaternery

primary: AA sequence 

secondary: alpha helix and beta sheets

Tertiary: supersecondary 

  • motifs and domains 

Quaternary: tertiary put together 

Term
Domains
Definition

Domains: discrete, independently folded, compact units 

may contrast of motifs

  • classified by structure 
  • bound to each other through weak interactions formed by AA side chains on the surface of each domain 
  • Fold: combination of secondary structures that form the con
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