Term
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Definition
-bacterial virus
-basis for recombinant dna technogolies
-clinical applications: bacterial typing, microbicides, etc
-provide means for genetic exchange: spread of antibiotic resistance, etc
-lysogenic conversion:alters bacterial virulence properties
****** insight into basic molecular biological processes: gene regulation, dna replication and repair, etc |
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Term
| culture and study bacteriophages by |
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Definition
plaque assay:
add serial dilutions of potential lysates to molten agar containing bacteria
plate mixture onto solid agar culture medium
incubate overnight for a progressive infection |
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Term
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Definition
1. double stranded dna (t-phages, lamda)
2. single-stranded dna (m13)
3. double-stranded rna
4. single-stranded rna |
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Term
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Definition
1. icosohedral- dome
2. helical- spiral staircase |
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Term
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Definition
lyse and kill the infected bacterial cells
-adsorption
-penetration
-replication |
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