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Bacteriology Lab Midterm
N/A
52
Microbiology
Undergraduate 4
11/09/2014

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Cards

Term

Give the Gram reaction and morphology

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Definition
Gram-positive cocci (sarcina - tetrads)
Term

Give the Gram reaction and morphology

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Definition
Gram positive bacilli
Term

Gram reaction and morphology

[image]

Definition
Gram negative coccobacilli
Term

Gram reaction and morphology

[image]

Definition
Gram negative bacillus
Term

Name the type of hemolysis for A

[image]

Definition
beta hemolysis
Term
Name the type of hemolysis for B
[image]
Definition
alpha hemolysis
Term
Name the type of hemolysis for C
[image]
Definition
gamma hemolysis
Term
True or False This catalase test most likely identifies Streptococcus pyogenes
[image]
Definition
False All Streptococcus species are catalse negative
Term
What reagent was added to cause this reaction with catalase?
[image]
Definition
hydrogen peroxide (H2O2)
Term
For this bile solubility test, name the reagent added and the most likely organism we studied.
[image]
Definition
sodium deoxycholate
Streptococcus pneumoniae
Term
Which number would identify Staphylococcus epidermidis from Staphylococcus aureus in this coagulase test?
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Definition

The correct answer is #2.

Staphylococcus epidermidis is coagulase negative

Term

#1 is the correct coagulase test for Staphylococcus aureus

True or False
[image] 

Definition
True Staphylococcus aureus is coagulase positive
Term
Which letter is indole negative?
[image]
Definition
B
Term
Which letter is indole positive?
[image]
Definition
A
Term
Look at the two MIO tubes labeled #1. The second number always indicates the addition of Kovac's reagent. Interpret tubes #1.
[image]
Definition

Indole positive

Non-motile
Ornithine negative 

Term
Look at the two MIO tubes labeled #2. The second number always indicates the addition of Kovac's reagent. Interpret these tubes.
[image]
Definition
Indole negative 
Motile
Ornithine positive
Term

Look at the two MIO tubes labeled #3. The second tube indicates the addition of Kovac's reagent. Interpret the results

[image]

Definition

Indole positive

Motile

Ornithine positive

Term

Which tube is positive for bile esculin?

[image]

Definition
the right tube shows the positive reaction for bile esculin
Term
For this oxidase test, which letter would most appropriately match the reaction of E. coli?
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Definition

E. coli and most other enterics are oxidase negative

Term
Which letter shows an oxidase positive reaction?[image]
Definition
A
Term
Which tube is positive or demonstrates survival in 6.5% NaCl?
[image]
Definition
the left tube. Turbidity in salt = positive reaction
Term
The tube the arrow points to indicates what hippurate reaction? What Strep organism is associated with this reaction?
[image]
Definition
The right tube shows a positive hippurate reaction
Streptococcus agalactiae
Term
True or False This CAMP test is positive[image]
Definition
True
Term
What two organisms are involved in this reaction?
[image]
Definition
Staphylococcus aureus and Streptococcus agalactiae
Term
This organism shows what type of reaction for DNase?
[image]
Definition
positive
Term
On this Mannitol Salt Agar, the most likely organism present is:___________
[image]
Definition
Staphylococcus aureus
Term

True or False Staphylococcus saprophyticus demonstrates the following reactivity with Novobiocin

[image]

Definition

False

Staphylococcus saprophyticus is resistant to Novobiocin, but S. aureus and S. epidermidis are susceptible.

Term

True or False Streptococcus pyogenes demonstrates the right reaction for PYR
What reagent was added?

[image] 

Definition
1) True
2) p-dimethylaminoacinnamaldehyde
Term
The most likely identity of this organism is: _______
It is Bacitracin ______________
[image]
Definition

Streptococcus pyogenes

susceptible

Term
The identity of an organism that grows in 6.5% NaCl, Catalase =, Bile Esculin +, and PYR + is:
Definition
Enterococcus spp.
Term
A P disk was placed on this plate. 
1) What is the other name for this antibiotic?
2) What is the most likely organism from Streptococcus?
[image]
Definition
1) Optochin
2) Streptococcus pneumoniae or pneumococcus
Term
Interpret the second tube: Red slant/red butt
[image]
Definition

No fermentation. Peptone catabolized aerobically and anaerobically with alkaline products.

Alk/Alk

Term

Interpret the third tube: red slant and yellow butt

[image]

Definition

Glucose fermentation with acid production. Proteins catabolized aerobically (in the slant) with alkaline products

Alk/A

Term
Interpret the fourth tube: yellow slant and yellow butt
[image]
Definition
Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt. Gas production
A/(A)
Term
Interpret the fifth tube
[image]
Definition
Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt. Gas production and sulfur reduction.
A/(A)/+
Term
Interpret the sixth tube
[image]
Definition
Glucose fermentation with acid production. Protein catabolized aerobically (in the slant) with alkaline products. Gas production and sulfur reduction occurred.
Alk/(A)/+
Term
Name three organisms with this IMViC reaction:

++--
Definition

E. coli

Edwardsiella tarda

Morganella

Term
Name three organisms with this IMViC reaction:
- - + +
Definition
Enterobacter aerogenes
Enterobacter cloacae
Klebsiella pneumoniae
Term
True or False This organism requires hematin for growth.
The most likely organism is?
[image]
Definition
True. This organism requires hematin (aka X factor, hemin) for growth. It's most likely Haemophilus ducreyi
Term
What three organisms demonstrate this pattern of growth for X and V factors?
[image]
Definition
Haemophilus influenzaeHaemophilus influenzae (aegypticus), and Haemophilus haemolyticus
Term
What does this β-lactamase result indicate?
[image]
Definition
The organism produces β-lactamase making it resistant to β-lactam antibiotics
Term
Interpret these results:
[image]
Definition
The organism is able to do both oxidation and fermentation (or fermentation only).
Term
Interpret these results:
[image]
Definition
The organism is able to oxidize sugars
Term
Interpret these results:
[image]
Definition
The organism is asaccharolytic or nonsaccharolytic. Doesn't perform sugar metabolism
Term
What reagent was added to cause the reaction in the top tube for the phenylalanine deaminase test?
 [image]
Definition
ferric chloride (FeCl3)
Term
What Neisseria species does this?
[image]
Definition
Neisseria gonorrhoeae
Term

What Neisseria species does this?

[image]

Definition
Neisseria lactamica
Term

What Neisseria species does this?

[image]

Definition
Neiseeria meningitidis
Term
Which tube demonstrates citrate positive reaction?
[image]
Definition
The tube with the deep dark blue-colored agar
Term
What is the name of this phenomenon and what organisms are involved?
[image]
Definition

satellitism
Staphylococcus aureus is the main, large inoculated colony

Haemophilus make up the tiny colonies because they require the V factor produced from S. aureus 

Term

Which test tube is positive for methyl-red?

[image]

Definition
Letter B
Term
Voges-Proskauer Test Results
Definition
[image]
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