Term
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Definition
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Term
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Definition
| determine motility: stabs |
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Term
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Definition
| to observe colonial morphology, isolate pure cultures, bulk growth |
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Term
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Definition
- Ability of cell to move from one location to another
- Requires presence of locomotive structure
- Sheath-gliding bac
- axial filament in spirochetes
- flagella
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Term
| Direct Determination of motility |
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Definition
| fix and stain cells to id number and position of flagella |
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Term
| Indirect determination of Motility |
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Definition
- Inoculate semi-solid motility agar and watch for migration away from area inoculated
- Growth in areas not initially inoculates indicates bac. capable of movement.
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Term
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Definition
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Term
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Definition
| Can tell the exact place of where it was stabbed. |
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Term
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Definition
| Not a quantitative method |
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Term
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Definition
Allow enumeration of cells and isolation
Cultures serially diluted and the transferred to agar |
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Term
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Definition
| Diluted culture pipetted on top of agar and spread over surface with glass rod |
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Term
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Definition
| Diluted cULTURE pipetted into warm agar and stirred, then poured into sterile dish |
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Term
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Definition
| all colonies have same morphological features |
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