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Bacteriological Media
Lab
17
Microbiology
Undergraduate 2
03/01/2011

Additional Microbiology Flashcards

 


 

Cards

Term
Selective Media
Definition
Inhibits growth of some microbes while allowing growth for another
Term
Differential Media
Definition

Used to discriminate between closely related org. with differences in characteristic growth patterns and biochem. changes

 

pH indicators

Term
Differential Media pH indicators
Definition
  • Acid Production
  • H2S production
  • Indole Production
  • Coagulase Activity
Term
Differentially Selective Media
Definition
  • Combines aspects of both selecective and diff
Term
Staphylococcus 11O (S110) Agar: Selective
Definition
  • Selects for NaCl
  • iF Staphyloccocus present, will grow
Term
Triple Sugar iron tubes: Differential
Definition
  • Differential: contains phenol red as pH indicator
  • Butt Reactions: glucose fermentation, H2S production
  • Slant reaction:lactose and/or sucrose fermentation
Term
Triple Sugar Iron Tubes: + AND -
Definition
  • Gas: Bubble- positve, no bubble- neg
  • H2S: black in butt: +, not black:-
  • K= alkaline, A= acidic
    • Finish this card later
Term
Sulfur Indole Motilty Tubes: Differential
Definition
  • Fe is indicator of H2S
  • Kovac's must be added after incubation to observe indole production.
    • Amber= indole neg
    • Red= indole pos
  • Semisolid medium=allows us to observe motility
Term
Baird (BP) Media: Selective and Differential
Definition
  • Selective: tellurite and LiCl
  • Differential: tellurite and egg yolk
    • Coag +: clear zone
    • Coag -: no clearance
    • tellurite reduction: black
  • Staph grows on these
Term
Mannitol Salt Agar: Selective and Differential
Definition
  • Selective: NaCl (if it grows, contains staph)
  • Differential: pheno red as indicator
    • Neutral/Alkaline=red= mannitol -
    • Acidic= yellow=mannitol +
Term
Mitis Salivarius Agar: Selective and Differential
Definition
  • Selective: crystal violet and tellurite
  • Diff: trypan blue and tellurite
    • Strep= blue
    • Entero=dark blue/black
Term
Eosin Methylene Blue Agar: Selective and Differential
Definition
  • Selective: methylene blue and eosin Y dyes
  • Diff: methylene blue and eosin Y are pH indicators
    • n-a: opaque growth
    • acidic=purple/black
    • highly acidic= green/metallic sheen
  • Acidic=lactose positive
Term
MacConkey Agar: Selective and Differential
Definition

Selective: crystal violet and bile salts

Differential: neutral red pH indicator

  • n-a: opaque growth
  • acidic=pink growth
  • Acidic= lactose positive
  • basic= lactose neg
Term
Salmonella Shigella Agar: Selective and Differential
Definition
  • Selective: sodium citrate, bile salts, brilliant green
  • diff: neutral red as pH indicator, Fe to indicate H2S
  • N-a: opaque growth (lactose neg)
  • Acidic- pink growth (lac positive)
  • H2S=black
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