Term
| What is the difference between in vitro and in vivo? |
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Definition
| In vitro is cultivation and/or testing of microorganisms within laboratory vessels and in vivo is within living organisms |
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Term
| What is the percent of agar in solid media, semi-solid media, and liquid broth? |
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Definition
Solid - 15-20% agar
Semi-solid - 0.2-0.4%
Liquid - None |
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Term
| Describe the three hemolytic patterns. |
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Definition
| Beta, Alpha and Gamma hemolysis |
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Term
| Name two purposes of Thioglycollate 135C Broth (Thio) |
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Definition
1) Supports growth of anaerobic, aerobic, microaerophilic, and fastidious microorganisms
2) Holding medium |
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Term
| What is the colony morphology for BAP? |
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Definition
| Hemolytic pattern, size and color |
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Term
| Which medium is commonly used for primary isolation of clinical specimens? |
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Definition
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Term
| What is the colony morphology on SCA? |
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Definition
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Term
| What type of bacteria is isolated on Colistin Nalidixic Acid Agar(CNA)? |
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Definition
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Term
| What is the colony morphology for the MacConkey Agar (MAC) Plate? |
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Definition
| size and color (use RLF or NLF for color report) |
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Term
| What is the colony morphology for the Hektoen Enteric Agar (HEK) Plate? |
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Definition
| size, color (yellow or blue-green), and H2S |
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Term
| List the carbohydrates that can be utilized in XLD |
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Definition
| Xylose, Lactose and Sucrose(Dextrose) |
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Term
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Definition
| A population of microorganisms cultivated in a medium of nutrients |
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Term
| What is Bacterial Culture Media? |
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Definition
| Nutrients used to support the growth of bacteria in a laboratory (in vitro) |
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Term
| What is Artificial Culture Media? |
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Definition
| Media composed of non-living organic and/or inorganic chemicals |
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Term
| What is Synthetic (chemically defined) media? |
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Definition
| All components and concentrations are known |
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Term
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Definition
| Contains some ingredients of unknown composition and/or concentration |
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Term
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Definition
| Contains some ingredients of unknown composition and/or concentration |
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Term
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Definition
| Contains some ingredients of unknown composition and/or concentration |
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Term
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Definition
| Contain some ingredients of unknown composition and/or concentration |
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Term
| What is Viral Cultivation Media? |
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Definition
| Composed of pH balanced basal salt solutions containing serum as needed to support the growth of the living tissue cells |
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Term
| What are the 5 purposes for Culture Media? |
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Definition
1) Cultivation of bacteria
2) Isolation of bacteria
3) Selection of particular bacterial types
4) Differentiation of bacteria
5) Obtain sufficient growth for identification |
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Term
| What is the percent agar for a solid media? |
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Definition
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Term
| What is the percent agar for Semi-solid agar? |
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Definition
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Term
| What is the purpose (3) of Basal media? |
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Definition
1) General purpose
2) Supports most bacterial growth
3) Basic constituents for other media |
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Term
| What is the purpose (4) of Enriched media? |
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Definition
1) Supports the growth of most fastidious bacteria
2) Contains additional nutrients
3) Primarily isolation
4) Differential testing |
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Term
| What is the purpose (2) of Selective media? |
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Definition
1) Supports the growth of particular microorganisms
2) Prevents the growth of certain bacteria |
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Term
| What is the purpose (2) of Differential media? |
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Definition
1) Distinguishes between different groups of bacteria on the basis of biological characteristics
2) Variation in colony |
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Term
| What is an example of a Basal Media? |
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Definition
| Soybean Casein Digest Agar/Tryptic Soy Agar (SCDA/TSA) |
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Term
| What is an example of a Semi-Enriched Media? |
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Definition
| Thioglycollate 135C Broth (Thio 135C) |
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Term
| What is the purpose (3) of the Blood Agar Plate (BAP)? |
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Definition
1)Enriched and differential medium suitable for the cultivation of non-fastidious and most fastidious bacteria
2) Differentiates bacteria based on interpretation of the hemolytic pattern
3) Primary isolation of most medically significant bacteria |
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Term
| What is the purpose (2) of the Supplemented Chocolate Agar (SCA)? |
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Definition
1) Highly enriched medium which supports the growth of most bacteria and highly fastidious bacteria
2) Required for the recovery of many species of Haemophilus and Neisseria |
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Term
| What is the purpose of Colistin Nalidixic Acid Agar (CNA)? |
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Definition
| Selective and enriched medium used for the recovery and isolation of gram-positive bacteria |
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Term
| What is the purpose (2) of the Modified Thayer-Martin (MTM) or Martin-Lewis Agar? |
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Definition
1) Enriched and selective medium
2) Isolation of pathogenic Neisseria spp |
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Term
| What is the purpose (3) of the MacConkey Agar (MAC)? |
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Definition
1) Selective and differential medium
2) Primary isolation of gram-negative bacilli
3) Differentiation of GNB on basis of lactose fermitation |
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Term
| What is the purpose (3) of the Hektoen Enteric Agar (HEK)? |
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Definition
1) Selective and differential medium
2) Isolation of gram-negative bacilli
3) Differentiation of GNB on the basis of salicin, lactose, and/or sucrose fermentation |
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Term
| What is the purpose (4) of the Xylose-Lysine-Deoxycholate Agar (XLD)? |
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Definition
1) Selective and differential medium
2) Isolation of gram-negative bacilli
3) Differentiation basis on xylose, lactose, and/or sucrose fermentiation
4) Medium is designed to support the growth of enteric pathogens |
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Term
| What is the purpose (3) of Thiosulfate-Citrate-Bile Salts-Sucrose Agar (TCBS)? |
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Definition
1) Selective and differential medium
2) Provides isolation of Vibrio spp
3) Differentiates based on sucrose fermentation |
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Term
| What is the purpose of Campylobacter Blood Agar Plate (Campy BAP)? |
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Definition
| Enriched and selective for the isolation of Campylobacter species |
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