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B2 Topic-Enzymes
Enzymes topic at GCSE level
73
Biology
10th Grade
04/30/2013

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Term
What are proteins made of?
Definition
Long chains of amino acids
Term
What can produce different proteins?
Definition
Different arrangement of the molecules.
Term
What is the function of proteins- in antibodies?
Definition
The chemicals of antibodies are produced by white blood cells to fight disease
Term
What is the function of proteins- Hormones?
Definition
They are chemical messengers which are transported around the body through blood.
Term
What are the functions of proteins-Enzymes?
Definition
These are biological catalysts which make reaction speed up
Term
What is the function of proteins in terms of structural?
Definition
They form muscles and tendons
Muscles- contract to move bones
Tnedons- attach muscles to bones
Term
What is activation energy?
Definition
The minimum amount of energy that particles need to react.
Term
What can the rate of chemical reactions increase by?
Definition
Temperature, concentration and presence of a catalyst
Term
Why does increase in temperature increase the rate of reaction?
Definition
Increasing the temperature of reacting particles will increase the rate of reaction as the particles have more kinetic energy and will collide more often with enough energy to react.
Term
Why does increasing the concentration of particles increase the rte of reaction?
Definition
There are more particles present and therefore there is a higher chance that they will react with enough energy to make the reaction take place.
Term
What are catalysts?
Definition
Catalysts are special chemicals which increase the rate of chemical reactions while remaining itself unchanged.
Term
How do catalysts work?
Definition
They work by bringing reacting particles together (attracting them) and lowering the activation energy needed for the particles to react.
Term
Reactions in what type of cell need catalysts and why?
Definition
In living cells as they have to be carried out at particular rates otherwise life would not be sustained.
Term
What are biological catalysts called?
Definition
Enzymes
Term
What are enzymes used to catalyse?
Definition
Building up large molecules rom smaller ones e.g. protein synthesis and photosynthesis
Breaking down large molecules into smaller ones e.g.respiration and digestion
Changing one molecule into another
Term
What are enzymes made of?
Definition
Large protein molecules made up of long chains of amino acids.
Term
What is the active site made up of?
Definition
Long chains of protein molecules that are folded and coiled to produce a specific shape who enables other molecules to fit into the enzyme.
Term
What will enzymes only react with?
Definition
A specific substrate
Term
Why will an enzyme only react with a specific substrate?
Definition
The active site is complementary in shape to the substrate so the substrate fits into the active site. This lowers the activation energy.
Term
Why is animal tissue more active than plant tissue?
Definition
Animal tissue is more metabolically active
Term
What is the lock and key hypothesis?
Definition
The substrate fits into the active site on the enzyme due to its complementary shape.
The active site changes shape slightly and puts pressure on the substrate molecule, this reduces the activation energy.
Products are forms in the active site
The products are released from the active site which is then ready to receive the next substrate molecule
Term
Define: Catalyst
Definition
A substance which changes the rate of a chemical reaction without being change itself.
Term
Define: Enzyme
Definition
A biological catalyst
Term
Define:Active SIte
Definition
The special site in the structure of an enzyme where the substrate binds
Term
Define: Optimum pH
Definition
The pH which the enzymes work best at.
Term
Define: Intracellular Enzymes
Definition
These are enzymes that are involved in chemical reactions inside body cells
Term
Define: Extracellular Enzymes
Definition
Enzymes which work outside body cells
Term
What are the functions of the gut?
Definition
To help break down food into smaller pieces and increase the surface area for enzymes to work on.
Mixes food with digestive juices so that enzymes come into contact with the food molecules.
Muscles in the wall of the gut move food from one area to the next in a process called peristalsis
Term
What is the main purpose of digestion?
Definition
To turn large insoluble food molecules into smaller molecules that can be absorbed into the blood stream and be carried around the blood stream to all areas of the body where they can be used
Term
What are starch molecules made up of?
Definition
Long chains of glucose molecules
Term
Where is amylase produced?
Definition
In the salivary glands and in the pancreas
Term
What does amylase do?
Definition
It breaks down starch by breaking down the bonds holding the glucose molecules together .
Term
Why does amylase convert starch into glucose?
Definition
Glucose molecules are small enough to pass through cells which form the gut wall and then into the blood stream trough the blood vessel wall.
Term
Where is pepsin found?
Definition
In the stomach
Term
Which organ makes enzymes?
Definition
The pancreas
Term
What pH is the stomach?
Definition
Acidic conditions
Term
What pH is the small intestines?
Definition
Slightly alkaline due to bile
Term
Where is the main site of digestion?
Definition
In the Small Intestines
Term
What is protein broken down into?
Definition
Proteases such as pepsin brak them down into amino acids
Term
What are carbohydrates broken down into?
Definition
Carbohydrases such as amylase break them down into glucose
Term
What are fats broken down into?
Definition
Lipases break the down into fatty acids and glycerol
Term
What tests the presence of glucose?
Definition
Benedict's Regent. It turns blue to orange if glucose is present.
Term
How would you test for the presence of starch?
Definition
You add iodine and if starch is present it will turn from red to blue/black.
Term
In what conditions is optimum for salivary amylase?
Definition
Neutral. Slightly alkaline- Approximately, pH7
Term
What is the name of the enzyme found in the stomach and in what conditions does it work best in?
Definition
Pepsin (gastric protease) works in very acidic conditions such as pH 2 due to hydrochloric acid being produced in the stomach to kill any bacteria
Term
What enzymes are found in the early part of the small intestines? In which conditions do they work best in?
Definition
Pancreatic amylase, pancreatic protease, and pancreatic lipase
The conditions are slightly acidic to begin with and then go alkaline as bile and pancreatic juices contain an alkaline solution to neutralise the stomach acid
Term
Which enzymes are found in the latter part of the small intestines?In which conditions do they work best in?
Definition
Carbohydrase, protease and lipase.
Due to the bile and pancreatic juices conditions are slightly alkaline at pH8
Term
Where is bile produced?
Definition
In the liver
Term
Where is bile stored?
Definition
In the gall bladder
Term
How is bile released into the small intestines?
Definition
Through the bile duct.
Term
What are the functions of bile?
Definition
Bile is alkaline so it helps to neutralise the acid from the stomach. This is because enzymes in the small intestines work best in alkaline conditions
Also, it emulsifies the dat in food.
Term
What is emulsification?
Definition
The break down of large fat droplets into smaller droplets. This gives a larger surface are for the lipase to work on
Term
True or False- Bile digests fats chemically
Definition
False, the bile breaks them down into smaller droplets. It is the lipase that breaks its down into fatty acids and glycerol
Term
The stomach produces gastric juices containing what?
Definition
Pepsin and hydrochloric acid
Term
Pepsin turns proteins into what?
Definition
Polypeptides
Term
The pancreas transfers enzymes to where
Definition
The duodenum
Term
With the addition of protease what do polypeptides turn into?
Definition
Amino Acids
Term
With the addition of salivary amylase what does starch turn into
Definition
Maltose
Term
With the addition of pancreatic amylase what does starch turn into?
Definition
Maltose
Term
With the addition of carbohydrase what does maltose turn into?
Definition
Glucose
Term
What is fat broken down into with the help of lipase?
Definition
Fatty Acids and Glycerol
Term
What is the main substrate for respiration
Definition
Glucose
Term
What are fatty acids and glycerol are used as?
Definition
A source of energy in respiration
to build up cell membranes
to make hormones
as an energy store in fat cells
Term
Which enzymes to biological washing powders contain?
Definition
Proteases and lipases
Term
Why are the use of enzymes an advantage?
Definition
They give a cleaner wash
They can be used at lower temperatures so the cloths are less likely to become damaged and less electricity is used
Term
Why are enzymes enclose in tiny capsules in washing powders?
Definition
So the enzymes do not harm you if handled
Term
What are some advantages of enzymes?
Definition
You do not need high temperatures or pressure for reactions
Enzyme based processes are cheap to run
Term
What are some disadvantages of enzymes?
Definition
Enzymes are denatured to temperatures musty be low
The pH must be kept low so the conditions are expensive to maintain
Enzymes are expensive
Pure enzymes use the substrate efficiently but they are more expensive.
Term
What enzyme is used to pre digest baby food?
Definition
Protease
Term
What are carbohydrases used for in the industry?
Definition
They break down starch into glucose syrup.
Term
What is isomerase enzymes used for in industry?
Definition
They change glucose into fructose which is sweeter and therefore useful in diet foods.
Term
What is pectinase used for in industry?
Definition
Pectinase digests the pectin in the cell walls of the plant cells and therefore allows fruit juice to be extracted more efficiently
Term
How do high temperatures effect enzymes?
Definition
Enzymes start to denature at higher temperature. This means the active site changes shape and the substrate will no longer fit into it.
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