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Asceptic Technique
Asceptic Technique
66
Medical
Professional
10/29/2012

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Term
Asepctic Technique
Definition
–Restrict microorganisms in the environment and on equipment and supplies from contaminating surgical wound
–Prevent normal body flora from contaminating the surgical wound
Term
External Barriers
Definition
–Skin and mucous membranes
–Usually impervious to most pathogenic organisms
Term
Inflammatory Response
Definition
–Prevents an invading pathogen from reproducing and possibly involving other tissue
–Occurs when tissues are injured by bacteria, trauma, toxins, heat, or any other cause. The damaged cells release chemicals including histamine, bradykinin, and prostaglandins. These chemicals cause blood vessels to leak fluid into the tissues, causing swelling. This helps isolate the foreign substance from further contact with body tissues
Term
Infection can take place in which body wall layers that have been incised?
Definition
•Superficial SSI – skin and tissue
•Deep incisional SSI – deep soft tissue, fascia, and muscle
•Organ or space SSI – any part of the anatomy other than the incision
Term
Contamination
Definition
–Presence of pathogenic microorganisms on or in animate or inanimate objects
–Term generally used to reference a specific object, substance, or tissue that contains microorganisms, especially disease-producing microorganisms
Term
Microorganism Shapes
Definition
•Classification – according to shape
–Cocci – round
–Bacilli – rod or tubular
–Spirilla
•Spiral or corkscrew shaped
•Have the ability to move on their own
Term
–Contains water
–Work is carried out here
Definition
Cytoplasm
Term
Maintains cell shape
Definition
Cell Membrane
Term
Nucleus
Definition
Contains genetic makeup of cell
Term
Slime layer
Definition
– Capsule
– Completely surrounds the cell
– Acts as a protective layer
– Has disease-producing power
–When using antiseptic it is used to penetrate this layer
Term
Factors for growth (temperature)
Mesophils
Definition
–37 0C
–Body temp
Term
Factors for growth (temperature)
Thermophil
Definition
–100 0C
–Can survive at boiling point of water
Term
Factors for growth (temperature)
Psychrophil
Definition
–0 0 C
–Below freezing
Term
Factors for growth
– Oxygen(O2)
Definition
•Aerobic – need O2 to grow and survive
•Anaerobic – can’t grow if O2 is present
•Facultative – can survive with or with out O2
Term
Bacteriostatic Agent
Definition
Inhibit growth and reproduction of bacteria without killing them
Term
When environmental conditions become unfavorable some bacteria may form a hard outer shell to help them survive
•They are called?
Definition
bacterial endospores
Term
Most are bacterias are harmless except
Definition
Anthrax (Bacillus Anthracis) and Tetanus (Clostridium Tetani)
Term
Fungi reproduce by
Definition
forming spores
Term
Fungi spores can spread through
Definition
contact, float on the water or float in the air
Term
Difference between yeast and mold is
Definition
yeast is non-filamentus (no strands or hair like projections)
Term
Simplest form of life
Definition
Viruses
–Much smaller than bacteria
–Need an electron microscope
–Able to pass through bacteria filters
Term
First line of defense
Definition
Skin
Term
Signs of inflammation
Definition
– Pain
– Redness (Erythema )
– Immobility (Dysfunction of area of inflammation)
– Swelling
– Heat (warm to the touch)
Term
Local tissue reaction due to injury (ChESTER)
• Causes
Definition
– Chemicals
– Excessive heat
– Sun
– Trauma
– Electricity
– Radiation
Term
Septicemia
Definition
The presence of bacteria in blood and is often associated with sever infection
Term
Sterilization
Definition
Complete elimination of microbial viability
Term
Two main types of sterilization procedures used in hospitals
Definition
–Steam
–Chemical sterilization (cold sterilization)
Term
Safest most practical means of sterilization
Definition
steam
Term
Holding time and temp for steam sterilization
Definition
Holding time/temp – 250°F (121°C) for 30 minutes
Term
Three types of steam sterilizers
Definition
•Gravity or downward displacement sterilizer
•Pre-vacuum or mechanical air removal, high temperature sterilizer
•High speed pressure sterilization, (flash type)
Term
5 phases of steam sterilization
Definition
–Loading phase
•package, packing and loading
–Heating phase
•when steam penetrates the article
–Destroying phase
•when microbes are destroyed
–Drying and cooling phase
–Testing phase
 checking the sterilizer calibration
Term
•Chemical sterilization (cold sterilization)
–Two types are?
Definition
•Gas (Ethylene Oxide EtO)
•Liquid (Cidex)
Term
Disinfection
Definition
–The process of destroying or inhibiting disease-producing microorganisms outside the body
–This process may destroy tubercle bacilli and inactivate hepatitis viruses but will not usually kill resistant bacterial spores
Term
Antiseptic
Definition
Chemical used to disinfect or inhibit microorganisms in an animate object (skin, hand)
Term
Disinfectant
Definition
Chemical used to disinfect inanimate object (outside the body)
Term
Types of disinfectants
Definition
–Halogens and halogen compounds (iodines, chlorines)
–Heavy metals
•Poor disinfectants (thimersols-found in salines, mercury)
–Phenols (C6H5OH)) used primarily as intermediate to low level disinfectant for environmental disinfection. May leave residue and cause tissue damage
– Alcohols (isopropyl 70%, ethyl alcohol 90%)
•More used as antiseptic than disinfectant
Term
3 antimicrobial agents used are?
Definition
–Povidone-iodine comples
–Chlorhexdine gluconate (CHG)
–Parachloro-mextaxylenon (chloroxylenol, PCMX) for those with sensitive skin
Term
How many strokes when scrubbing the nails?
Definition
30
Term
How many strokes when scrubbing the skin areas?
Definition
20
Term
Single-Use disposable drapes prevent bacterial penetration and fluid breakthrough, also known as what?
Definition
strikethrough
Term
Damaged cells release chemicals including histamine, bradykinin, and prostaglandins which cause what?
Definition
These chemicals cause blood vessels to leak fluid into the tissues, causing swelling. This helps isolate the foreign substance from further contact with body tissues
Term
What are the characteristics of Microorganisms?
Definition
–Unicellular units (one cell)
–Protista (plant kingdom)
–Microscopic
–Protoplasm
Term
What is a bacteria cell composed of?
Definition
–Bacteria cell is composed of protoplasm and 4 parts
•Cell membrane
•Cytoplasm
•Nucleus
• Slime layer
Term
What are the factors of microorganism growth?
Definition
– Oxygen(O2)
– Moisture
– Food sources
Term
What are food sources for microorganisms?
Definition
•Glucose • Phosphorus
•Proteins • Sulfur
•O2 • Nitrogen
Term
At what rate do microorganisms reproduce?
Definition
Reproduce at a fast rate hours/days
Term
What are the microorganism cell groupings?
Definition
–Diplococci – 2 paired (dipole) oo bb
–Streptococci
•Chains
•Cause: Rheumatic fever, Endocarditis
–Staphylococci
•Masses • Usually harmless
•Clusters • Most prevalent in the skin
Term
Which microorganism is more prevalent in the skin?
Definition
Staphylococci
•Masses • Usually harmless
•Clusters
Term
Which microorganism causes: Rheumatic fever and Endocarditis?
Definition
Streptococci
•Chains
Term
Which bacteria will form endospores
Definition
Bacillus, Cocci and Clostridium
Term
70% of yeast infections are caused by what?
Definition
Candida
Term
byproduct of alcohol and CO2
Definition
Fermentation process
Term
Live in the soil usually in decomposed organic matter
Definition
Mold
Term
traps pathogens, dirt, and particulate matter so that they can be sequestered and eliminated by the body
Definition
Mucus
Term
•Rich in blood flow
•May kill some bacteria
Definition
Liver
Term
synthesizes antibodies
Definition
Spleen
–Removes antibody-coated bacteria along with antibody-coated blood cells
Term
What are some parts of the immune system?
Definition
tonsils, lymph nodes, appendix, spleen and thymus
Term
Specialized cells that patrol throughout your body looking for pathogens
Definition
white blood cells
Term
A collection of pus beneath the skin
Definition
Boil
Term
Mass collection of boils
Definition
Carbuncle
Term
Dead bacteria and tissue
Definition
Pus
Term
Used only for materials that are heat sensitive
Definition
Ethylene Oxide (EtO)
Term
Once activated Cidex is good for how many days?
Definition
14 days
Term
Oldest for liquid sterilization
Definition
Formaldehyde
Term
What is the shelf life if the item is plastic sealed?
Definition
6 months to a year
Term
The ‘Gold Standard’ for surgical asepsis
Definition
Hand scrub
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