Shared Flashcard Set


Application of molecular techniques

Additional Biochemistry Flashcards




__________ __________ is used to detect VNTR (variable number of tandem repeats) in fragile X syndrome.

a. Southern Blotting
b. Western Blotting
c. Northern blotting
d. PCR
a. Southern blotting

cp page 433
If the causative gene is known, and you want to find out what specific mutation has occurred, which of the following is appropriate testing?

a. Sanger sequence
b. Karyotyping
c. CGH array, or CGH
d. Whole exome sequencing
Sanger Sequence would be appropriate. You have the gene, but don't know what mutation has occurred.

CP 449
A method of testing for Hepatitis C virus includes creating a PCR product, digesting it with enzymes, and analyzing the pattern to look for a specific genotype. This is characteristic of what molecular method?

a. Southern blot
c. ASO
Restriction fragment length polymorphism

cp page 447.
In FISH (fluorescence in situ hybridization), two probes are used detect EGFR2 that amplifies the cancer.

These are:

________ __________ _________ (CEP)
a __________ __________ _________(LSI)
Centromere enumeration probe


Locus Specific Identifier

cP page 443.
A physician would like to determine the global pattern of gene expression in two different types of
tumor cells in order to develop the most appropriate form of chemotherapy for each patient. Which
of the following techniques would be most suited for this purpose?
A. Northern blot
B. Southern blot
C. Western blot
D. Comparative genomic hybridization
E. Microarray gene expression profiling
e. Microarray gene expression profiling

CP 440
PCR for epidermal growth factor 1 (EGFR1) is used to test for this form of cancer, ______ cancer, which has an 85% mortality rate. This makes this the most lethal cancer in the US.
PCR --> EGFR1 (Tyrosine kinase) --> Looks for lung cancer

CP p 441
You wish to analyze the pattern of genes expressed in a breast cancer in order to identify potential
differences to normal breast tissue cells. Which of the following enzymes is most useful to you in
your anticipated studies?
A. DNA ligase
B. DNA polymerase II
C. Taq DNA polymerase
D. Reverse transcriptase
E. Restriction endonucleases
D. Reverse Transcriptase

Practice test 2
A 4-year-old male presents to his pediatrician. His mother expresses concern that the child has
been regressing since the previous year. Upon examination, the patient has regressed in his
milestones, specifically in motor and speech development. The patient was referred to a neurodevelopmental
specialist. Testing for Fragile X syndrome using STR (short tandem repeat)
analysis of the FraX gene, for example, proved negative. Based on these findings and other
symptoms, the specialist believes the patient may have autism, which most likely represents a
multifactorial disorder involving inherited predisposition or genetic susceptibility interacting with
environmental factors. A reasonable next test is:
A. Southern blotting for the autism gene
B. Polymerase Chain Reaction (PCR) using centromere-specific primers for chromosome 5
C. Chromosomal microarray (array CGH) analysis
D. Fluorescence in situ hybridization (FISH) analysis using probes specific to chromosome
E. Chromosome analysis using solid staining
C. Array CGH

We went very detailed - using STR, and that didn't pick up anything, so now we step back and use a broader brush with Array CGH.... Looking for a large amplification or deletion.

CP page 449 and practice test
Which of the following relates to restriction fragment length polymorphism (RFLP) analysis?
A. Can be used to detect one base differences in DNA sequence
B. Detects differences in conformation of single strands of DNA
C. Requires the use of an RNA intermediate
D. Uses western blotting as part of the technique
E. Uses Sanger DNA sequencing as part of the technique
A. Can be used for 1 base differences

Very specific - so if you know the gene cause, AND the mutation cause, you can find out exactly where/what the problem is.

CP page 449
Which of the following is a function of restriction endonucleases in bacteria?
A. degradation of foreign DNA
B. enhancement of transcription
C. Participation in DNA repair
D. Participation in synthesis of lagging strand DNA
E. stimulation of recombination
A. Degradation of foreign DNA

Used as a tool - groundbreaking in 1971!!!

CP. p 416
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