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Antiseptics: Filter Paper Disk Method
Evaluation of antiseptics
19
Microbiology
Undergraduate 2
10/22/2011

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Term
Organic form of iodine ...
Definition
Betadine
Term
Eg. of skin bacteria ...
Definition

Staphilococcus aereus

(gram positive)

Term

What bacteria causes a

deadly form of food poisoning?

Definition
Clostridium botulinum
Term
What are antiseptics?
Definition
Substances that inhibit microbial growth or kill microorganisms and are gentle enough to the applied to living tissue.
Term
Eg. of antiseptics ...
Definition

Alcohol

Betadine

Term
Do antiseptics destroy endospores?
Definition
No!
Term
What are disinfectants?
Definition
Chemical agents that are applied to inanimate objects such as floors, walls and tabletops to kill microorganisms.
Term
How are disinfectants different from antiseptics?
Definition
They are harsher and damaging to living tissue.
Term
What are steriliants?
Definition
A group of disinfectants that can destroy all microorganism life, including endospores.
Term
What are steriliants also known as?
Definition
Sporocides
Term
Eg. of sporocide/steriliant...
Definition
ethylene oxide
Term
What are sanitizers?
Definition

Agents that reduce microbial numbers to a safe level,

but don't completely eliminate all microbes.

Term
In which industry are sanitizers used?
Definition
Food industry
Term
What are bacteriostatic agents?
Definition
Agents that inhibit the growth of bacterial cells, but don't kill them.
Term
What are bacteriocidal agents?
Definition
Agents that kill bacterial cells.
Term
What is a property that can influence the effectiveness of the agent under test conditions?
Definition
Solubility
Term
Can antiseptics be used as disinfectants?
Definition
Yes!
Term
What factors influence the size of the zone of inhibition produced by a chemical?
Definition

1. Molecular weight of agent

2. Rate of difussion in the agar

Term
How might the physical differences between gram-positive and gram-negative bacteria contribute to differences in chemical responses?
Definition
Gram negatives might be more resistent because they have extra protection.
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