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Ames Test
Micro lab exam
18
Biology
Undergraduate 3
11/10/2011

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Term
Ames Test
Definition
rapid and economically way of determining whether a substance is mutagen
Term
What is the idea of the ames test based on?
Definition
That most carinogen are mutagens and not all carcinogen are cancer causing agent.
Term
Mutagen
Definition
chemical substances that cause "induced mutations
Term
If a substance is a mutagen then what is the next step?
Definition
more tests are required to see if it is a carcinogen
Term
Prototroph
Definition
a bacterium that can make all amino acid on its own (can grow on minimal media)
Term
Auxotroph
Definition
a bacterium cannot synthesize their amino acid (grow on complete media)
Term
what type of bacteria did we use on the ames test?
Definition
S. typhimurium
Term
how many mutations does S. typhimurium have?
Definition
3 mutations
Term
what are the three mutations for S. typhimurium?
Definition
  1. His -
  2. uvrb
  3. rfa
Term
His -
Definition
cannot make histidine
Term
uvrb
Definition
cannot repair mutation
Term
rfa
Definition
cannot synthesize complete cell wall
Term
Carcinogens
Definition
all carcinogens compounds are mutagenic but not all mutagens are carcinogens
Term
how do we know if something is mutagenic?
Definition
must see if the substance we brought will increse the rate of reversion or back mutation
Term
Hist (-)→ Hist=
Definition
reversion mutation
Term
what will the chemical do to S. typhimurium?
Definition

THe chemical will permeat thru the cell wall of S. typhimurium 

 

Term
what was the negative control used?
Definition
Water
Term
what type of media did we use?
Definition
minimal media
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