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Advanced Bio Final
Advanced Bio!!!
31
Science
12th Grade
12/16/2008

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Cards

Term
Pegnancy Test and blood typing are examples of what type of biotechnology?
Definition
Monochromal Antibody
Term
Fermentation is an example of what type of technology?
Definition
Bioprocessing
Term
The technology used to inhibit the production of certain proteins is.....
Definition
Antisense
Term
What is genetic engneering?
Definition
using genetics and traits and applying them to research and development
Term
Name an agricultural application of biotechnology
Definition
food production
Term
Name a reproduction myth
Definition
Vital Heat
Term
An observable form of a trait is called
Definition
a phenotype
Term
What is Mendels law about different pairs of alleles seperated independent of each other during gamete formation?
Definition
Independent Assortment
Term
Name an experiment used to confirm DNA as gentic material
Definition
Hersey and Chase- they used radio active DNA to show bacterialphage changed genetic material
Term
What enzyme is required for transcription?
Definition
RNA Polymerase
Term
What enzyme is required for cDNA synthesis?
Definition
Reverse Transcriptase
Term
What enzyme is required for DNA replication?
Definition
DNA Polymerase
Term
What enzyme is required for the joining of pieces of nucleic acid?
Definition
DNA Ligase
Term
Name two DNA libraries and their difference
Definition
cDNA (specific, no introns) and genomic (entire)
Term
Two differences between PCR and RAPD
Definition
PCR is general while RAPD is specific
Term
Define Northern blot
Definition
RNA
Term
Define Western blot
Definition
protein
Term
define: probe
Definition
a short single strand peice of DNA that is labled and used in hybridization
Term
Define Aneuploidy
Definition
loss or gain of one or more chromosomes
Term
Define Euploidy
Definition
loss or gain of entire sets of chromosomes
Term
A triploid human will have how many chromosomes?
Definition
69
Term
What does VNTR stand for?
Definition
Variable Number Tandom Repeats
Term
What is the name of the transformation technique used to transfor bacteria?
Definition
Electroportation
Term
What is the name of the medium used to incubate the transformats for one hour?
Definition
SOC medium
Term
List the steps in DNA cloning
Definition
Do Like To See Patty
1) Digest
2) Ligate
3) Transform
4) Seperate Cells
5) Purify
Term
In Sanger sequencing, what causes DNA sythesis to be terminated at a specfic base?
Definition
Nucleotide triphosphates that lack oxygen at the 3' position of the sugar
Term
What are the steps of PCR?
Definition
1) Heat to 95-96 (break bonds)
2) Cool to 50-65 (Primers form)
3) Heat to 72 (elongation)
Term
What is the function of the enzyme Restriction Endonuclease?
Definition
aid in replication
Term
The chemical used to visualize DNA under UV light is
Definition
Ethidium bromide
Term
Name one tool used to transfer DNA
Definition
DNA gun
Term
Which direction does DNA move during electrophoresis?
Definition
towards the positive
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