Term
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Definition
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Term
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Definition
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Term
| State 2 potential hazards in a lab |
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Definition
| Splashes, hot objects, pathogenic organisms, vapours etc |
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Term
| State 2 control measures to reduce risk |
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Definition
| protective clothing, education, safety glasses, fume cupboards, tongs, mits etc |
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Term
| What is a Risk Assessment? |
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Definition
| Document designed to identify possible risks and the control measures to minimise them |
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Term
| What is a Linear Dilution? |
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Definition
| Dilutions in a linear dilution series differ by an equal interval. |
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Term
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Definition
| Dilutions in a log dilution series differ by a constant proportion. |
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Term
| What is a Standard Curve and what is it used for? |
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Definition
| Graph of known concentrations used to predict the concentration of an unknown solution |
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Term
| What is the purpose of a colorimeter? |
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Definition
| Measures concentration of a coloured solution or turbidity of a cloudy solution |
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Term
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Definition
| Controls pH during a reaction |
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Term
| How does a centrifuge separate materials? |
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Definition
| high speed spinning to separate materials according to density |
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Term
| Name the 3 types of chromatography. |
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Definition
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Term
| What is the basis of separation using paper and thin layer chromatography? |
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Definition
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Term
| What is the basis of separation using affinity chromatography? |
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Definition
| the ability of a molecule to bind to another molecule |
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Term
| What is the basis of separation using native gel electrophoresis? (three things) |
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Definition
| Molecular charge, shape and size |
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Term
| What is the basis of separation using SDS–PAGE gel electrophoresis? |
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Definition
| Size alone as the gel neutralises the charge and denatures the molecule. |
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Term
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Definition
| Isoelectric point, the pH at which a soluble protein has no net charge and will precipitate out of solution. |
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Term
| How are isoelectric points used to separate proteins. |
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Definition
| constructing a pH gradient. The protein will move across the gradient until it reaches the pH at which it has no net charge, where it precipitates out of solution. |
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Term
| What are Immunoassay Techniques (like ELISA) used for? |
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Definition
| detect and identify specific proteins. |
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Term
| What is Western Blotting? |
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Definition
| The separated proteins from the gel are transferred (blotted) onto a solid medium and specific antibodies are used with markers to identify the proteins |
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Term
| Name three possible markers used in immunoassays. |
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Definition
| Chemiluminescence, fluorescence and reporter enzymes. |
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Term
| Use of Fluorescence microscopy |
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Definition
| Locates specific molecules or structures by using antibodies which bind to them specifically. They carry fluorescent labels enabling their position to be identified. |
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Term
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Definition
| To view dissection, cells or whole organism slides. |
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Term
| Name two methods used to ensure sterilisation of glassware in microbiology. |
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Definition
| Chemical or heat sterilisation. |
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Term
| Purpose of growth factors in serum in microbiology. |
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Definition
| Promote growth and proliferation of animal cells. NB They are NOT and energy source. |
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Term
| What is the term in microbiology for the medium which favours a particular type of microbe? |
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Definition
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Term
| What piece of equipment is used to help count cells? |
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Definition
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Term
| What is the purpose of plating out serial dilutions of cell cultures? |
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Definition
| Allows colonies to be counted and cell concentration to be determined in the original source. |
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Term
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Definition
| Stains only dead cells and allows viable cells to be distinguished from total cell count. |
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