Term
| Mercaptoethanol, dithiothreitol, dithioerythreitol |
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Definition
| Cleaves disulfide bridges. strong reducing agent |
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Term
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Definition
| add S-CH2-Coo- to both ends of Sulfide bonds. prevents reformation. alkyalting agent |
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Term
| Sangers reagent beta-floruro-2,4, dinitrobenzene |
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Definition
| Reacts with N-terminal amino acid and labels it yellow. |
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Term
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Definition
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Term
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Definition
| cleaves off any residue @ C terminus except Pro, Arg, Lys. |
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Term
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Definition
| Only works on Arg and Lys |
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Term
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Definition
| Cleaves off C terminus of Arg and Lys |
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Term
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Definition
| Cleaves off C terminus of Phe, Tyr, Trp |
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Term
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Definition
| Like trypsin but attacks Arg more than Lys. |
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Term
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Definition
| C side of Glu, Asp in phosphate buffer |
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Term
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Definition
| Cleaves at C end of Asp, Glu, Leu, Phe, Trp, Tyr |
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Term
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Definition
| Cleaves at N-end of Leu, Ile, and Val |
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Term
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Definition
| Met identification, Cleaves at C-end |
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