Term
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Definition
| Protein in RBC binds O2 from Lungs and transport to tissue |
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Term
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Definition
| Muscle selves, stores reserve supply of oxygen in time of increase activity |
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Term
| Partial Pressure, arterial and venous |
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Definition
Concentration of O2, in mm Hg
Arterial 90 mm HG
Venous 40 mm HG |
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Term
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Definition
| the PO2 where 50% of the protein is saturated, lower PO2 shows higher affinity |
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Term
| Myglobin dissociation curve |
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Definition
| Hyperbolic affinity too high for venous function |
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Term
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Definition
Pure Hemoglobin is similar to Myoglobin
Hemoglobin with BPG is sigmoidal
Curve is result of co-operative behaviour, oxygen binding inreases affinity for future binding |
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Term
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Definition
| Oxy state, high binding affinity |
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Term
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Definition
| Low binding affinity (release of one O2 decreases by as much as 100x) |
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Term
| How does binging of O2 cause increased affinity in other subunits? |
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Definition
| 1) Oxygen changes the position of Iron in porphyrin, binding causes change to R state. Binding oxygen causes iron atom to shrink in size. Attached histine pulls F-helix of beta subunit. Beta2 subunit causes change in alpha1 subunit via electrostatitc interactions. Symmetry of molecule insures that change happen in other half too. |
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Term
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Definition
| Site of O2 binding; Fe2 is ferrous binds O2 FERROCIOUSLY BINDS o2 |
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Term
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Definition
Reduces ferric(+2) hemoglobin to ferrous (+3) hemoflobin, overwhelming the function of or underfunctioning methmoglobin causes related anemia Treat with reducing molecule like methylene blue
(ferrous binds O2) |
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Term
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Definition
| Biphosphoglycerate, helps stabilize t state by binding to positive residues of the beta subunits (sits in pocket between the two subunits) |
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Term
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Definition
| Distal v Proximal. Distal E7 stabilizes O2 and iron bonding. Proximal F8 causes change in F-helix |
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Term
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Definition
| 200x more affinity for hemoglobin than O2. Locks into R state. Wont release O2. |
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Term
| Increased in BPG caused by? |
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Definition
High Altitude
Airway obstruction
Congestive heart failure |
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Term
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Definition
| HbF (A2/G2) has low BPG affinity so higher O2 affinity, allows it to snatch up the moms O2 better. Gamma subunits have serines instead of positive His residues. |
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Term
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Definition
| Co2 and Hydrogen cause a rightward shift, stimulate O2 release via bohr effect |
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Term
| Bohr effect happens because |
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Definition
| salt bridges between His 146 and Asp 94 residues occurs when H is protonated, stabilizing the T-state. Increased H (low pH) stabilizes T state by causing salt bridge formation |
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Term
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Definition
Donates Protons (right shift)
Co2 reacts with N-terminal to create caramino group which stabilizes t-state by creating e-static ineraction betwen ab dimers. |
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Term
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Definition
Change of A6 from glutamic acid to valine in beta hemoglobin. Referred to as HbS causes aggregation of Hemoglobin S intofribril due to hydrophobic pocket. Eventually leads to hemolysis.
Chromosome 11 |
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Term
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Definition
| Abnormal Hemoglobin synthesis. Alpha thalasemias have to do with deficiency in a globin subunit and B for beta subunit. High frequency among people near the mediterranean. |
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