Shared Flashcard Set

Details

8/22 DNA Recombination
Crawford Theme1
11
Biology
Graduate
08/22/2011

Additional Biology Flashcards

 


 

Cards

Term
Rec A
Definition
General Recombination Enzyme, does initial base pairing then acts as an SSB
Term
Rad51/hsRad51
Definition
Human equivalents to RecA, General Recombination
Term
DNA Synapsis
Definition
Process by which DNA regions at site of crossing over recognize each other
Term
SSBs
Definition
Single strand binding proteins, keep DNA open for recombination
Term
Holliday junction
Definition
branched intermediate structure that results from DNA strand exchange
between DNA helices. A Chi structure is a Holliday structure intermediate that looks,
microscopically, like the Greek letter chi.
Term
Heteroduplex joint
Definition
– location where strands from two different DNA helices have basepaired
Term
BRCA1 and BRCA2
Definition
Accesory proteins that interact with RAD51, disrupting proper function is associated with breast and ovarian cancer
Term
Transposition and Site-specific recombination
Definition
genetic exchange that occurs at short
nucleotide stretches and requires little or no homology. Here, mobile genetic elements
("jumping genes") are used
Term
Transpositional recombination
Definition
- carried out by transposons (transposable elements),
mobile genetic elements that usually encode a transposase which insert into target DNA
without any homology requirement.
Term
Conservative site-specific recombination
Definition
– involves a short heteroduplex joint and
therefore short DNA sequence homology for recombination. A classic example is the
integration and exit of bacteriaphage lambda into and out of its target E. coli genome
Term
V(D)J (site-specific) recombination
Definition
– The combinatorial diversification of antibodies
resulting from the rearrangement of different combinations of antibody V, J, and D gene
segments in B-cells. This is mediated by an enzyme complex called the V(D)J
recombinase composed of RAG proteins. V(D)J recombination is also used to generate Tcell receptor diversity in T-cells
Supporting users have an ad free experience!