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Definition
The use of microrganisms, cells, or cell components to make a product.
ex. foods, antibiotics, and vitamins. |
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Term
| Recombinant DNA Technology |
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Definition
Insertion or modification of genes to produce things they do not naturally produce.
ex. e. Coli to make insulin |
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Definition
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Definition
| Self replicating DNA used to carry the desired gene to a new cell. (selection) |
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Definition
| Population of cells arising from one cell; each carries the new gene. Giving a characteristic to the gene it normally would not have. |
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Definition
Culture a naturally occuring microbe that produces the desried product.
ex. vector |
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Definition
| Mutagens cause mutations which result in a microbe desirable trait. |
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| Site Directed Mutagenesis |
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Definition
Changing the DNA.
ex. designer baby |
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Definition
| Cuts DNA and leaves sticky ends. |
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Definition
| Carry a chain of interest. You can pick what you want. |
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Term
| Polymerase Chain Reaction (PCR) |
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Definition
| Makes mutiple copies of DNA. 35 cycles take about 4 hours. After one cycle it doubles. Uses TAQ. |
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Definition
| Thermus auquaticus. Heats up DNA then cools. This is the replicatio of DNA. |
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Definition
| Inserts DNA electrically. |
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Definition
| Places naked DNA in solution. Cells take up the solution. Deals with temperature change. |
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Definition
| The cell wall is removed by enzymes. |
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Definition
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Definition
| DNA is directly inserted into an animal cell. |
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Definition
| A area where you can get plasmids or phages. |
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Term
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Definition
| Made by a DNA synthase machine. |
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Term
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Definition
| Made from mRNA by reverse transcription. |
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Term
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Definition
| Is used the most and is easily grown. You have to kill a lot of e. Coli to get insulin. |
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Definition
| Expresses Eukaryotic cells and is harder to grow. |
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| Protein portion of the pathogen. |
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Definition
| Can not get sick. Vaccine comes from the acutal virus. |
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Definition
| Small interfering RNA. Silences gene for a little bit. |
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