Term
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Definition
outer= freely permeable (basically cytosol)
inner- a specific protein carrier must be used to get across excetpt O2, H20, Co2. |
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Term
Matrix-
oxidation phosphorylation occurs- |
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Definition
| where TCA occurs and FA degraded. Inner membrane is where oxidative phosphorylation is located. |
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Term
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Definition
| increase SA w/out increaseing volume bc volume slows |
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Term
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Definition
| 4 enzyme complexes that pass e- along a series of redox rxns resulting in releases of energy which is used to pump H+ out of matrix |
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Term
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Definition
| complex 1 where it donates 2e- to coenzyme Q |
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Term
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Definition
| NADH + uniquinone +H+ -> NAD+ +ubiquinol |
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Term
| is ubiquinone oxidized or reduced? |
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Definition
ubiquinone is oxidised
quinol is reduced
**Know structual difference |
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Term
| what is the mobile carrier of e- that diffuses between complex 1 and 3 |
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Definition
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Term
| Once QH2 gets to complex 3 |
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Definition
| it passes e- on to cytochrome one at a time. cyt c can only accept one e- so the other e- cycles thru to cyt b. |
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Term
| What is the reducing agent at complex 3 |
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Definition
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Term
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Definition
ubiquinol +2cytc (Fe3+) -> ubiquinone +2cytc (Fe 2+) +2H+
Fe is reduced |
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Term
| what's covalently bound to cytc? |
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Definition
| Heme. Cystine side group adds across the dbl bond forming a covalent bond. |
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Term
| Difference in Mb heme for cytC heme |
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Definition
| Fe in Hb and Mb is to reversibly bind O2 and stays in Fe2+ state if oxized to +3 Mb would be unfunction. Cytc heme must undergo redox bc the cytc allows it to. Heme function depends upon the protein it is bound to (MB or cytc) |
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Term
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Definition
| one e- goes directly to cyt c while the other e- takes a circular detour via cytb and back to QH2 and then directly to cyt c. |
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Term
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Definition
| increases to 4 the number of H+ pumped from matrix to intermembrane space per 2e- passin along complex 3 |
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Term
| oxidizing agent at complex 4 |
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Definition
terminal step= Oxygen!
oxygen is the oxidizing agent that is reduced to H20 |
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Term
| what carries e- from 3 to complex 4 |
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Definition
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Term
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Definition
cytochrome oxidase
4 cytc (Fe2+) +O2 +4H+ -> 4 cytc (Fe3+) +2H20 |
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Term
| what e- enter at complex 2? |
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Definition
| e- from succinate DH (FADH2) are passed on to QH2 and thego |
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Term
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Definition
can't be reduced one e- at a time= ROS
O2 +1e-= O2- superoxide anion free radical
+2nd e-= peroxide HOOH = powerful oxidizing agent
+3rd e-= hydroxyl radical = most destructive
4th e- -> H20 |
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