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2.) Basic Cell Structure/Energy Transfer
ppt 2
34
Health Care
Undergraduate 3
03/02/2012

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Term
Cell Structure (3 parts)
Definition
Cell membrane, nucleus, cytoplasm (sarcoplasm in muscle cell)
Term
cell membrane
Definition
protective barrier between interior of cell and extracellular fluid, encloses cellular contents and allows regulation of substances in and out
Term
nucleus
Definition
contains genes that regulate protein synthesis which determine cellular composition and activity
Term
cytoplasm (sarcoplasm in muscle cell)
Definition
-fluid portion of cell

-contains organelles (mitochondria)
Term
molecular biology
Definition
study of the relationship between genes and the cellular characteristics they determine
Term
reactant
Definition
a substance that enters into and is altered in the course of a chemical reaction to yield a product
Term
substrate
Definition
a type of reactant that requires an enzyme to catalyze the reaction to yield products
Term
product
Definition
a substance produced from one or more other substances as a result of chemical change; substance found at the end of a reaction
Term
endergonic r(x)
Definition
require energy to be added

aka "uphill", nonspontaneous
Term
exergonic r(x)
Definition
release energy

aka "downhill", spontaneous

ex. breakdown of glucose
Term
coupled r(x)s
Definition
-liberated energy in an exergonic r(x) drives an endergonic r(x)

-all energy liberated during exergonic r(x)s is NOT captured during endergonic r(x)s: there is NOT always waste

-ex. if r(x) 2 C-->D is driven by r(x) 1 A-->B, then the absolute value of E2 < E1
Term
first law of thermodynamics
Definition
energy can neither be created nor destroyed; however the forms of energy can be exchanged
Term
second law of thermodynamics
Definition
the processes of energy exchange always go in a direction of a disordered form of energy
Term
E' IN (food) = ?
Definition
E' IN (food) = E' OUT (work) + E' OUT (heat) + E' stored (fat)
Term
properties of enzymes (catalysts)
Definition
-large 3D protein structures

-increase RATES of energetically favorable r(x)s

-do not change the direction of reactions

-do not change ratio of products and reactants
Term
the activity of enzymes is affected by:
Definition
1.) temperature: small rise in temperature INC activity

2.) pH: change in pH from an optimum level affects activity
Term
effect of temperature and pH on enzyme activity
Definition

- activity = rate at which substrate converted to product

 

-small rise in temp INC activity of enzymes -pH survival range of arterial blood is 7.0-7.8, with 7.4 considered normal

 

-as pH drops (INC acidity) from 7.8, enzyme activity first INC then DEC

 

-optimal activity of most enzymes occur within range of temp and pH encountered during exercise

 

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Term
types of mechanics of interaction for enzyme and substrate
Definition
-lock-and-key mechanism: substrate fits directly into binding site of enzyme

-induced fit: substrate induces a conformational change in the enzyme that allows the substrate to be properly positioned for catalysis
Term
what does an enzyme do?
Definition
lower activation energy of a r(x)
Term
oxidation-reduction r(x)s
Definition
reduction: acceptance of e- to a molecule

oxidation: loss of e- to a molecule

re-dox r(x)s are always coupled
Term
oxidizing agent
Definition
the molecule that can accept electrons in the oxidized state
Term
reducing agent
Definition
the molecule that can lose electrons in the reduced state
Term
transfer of hydrogen atoms rather than free electrons
Definition
H atom also contains 1 e-, so when a molecule loses a H it also loses an e-
Term
types of fuels for exercise (3)
Definition
-carbohydrates: glucose (stored as glycogen)

-fats: fatty acids (stored as triglycerides)

-proteins: not a primary energy source during exercise
Term
energy value of nutrients: fat
Definition
9.4 kcal (BC)
9 kcal (atwater)
Term
energy value of nutrients: CHO
Definition
4.2 kcal (BC)
4 kcal (atwater)
Term
energy value of nutrients: protein
Definition
5.65 kcal (BC)
4 kcal (atwater)
Term
definition of bioenergetics
Definition
the study of the flow of energy through living systems; involves the study of processes that make energy available within cells in the form of ATP
Term
components of bioenergetics (3)
Definition
-formation of ATP (phosphocreatine breakdown, glycolysis, oxidative formation of ATP)

-anaerobic pathways (do not involve O2, CP breakdown and glycolysis)

-aerobic pathways (require O2, oxidative phosphorylation)
Term
ATP (adenosine triphosphate)
Definition
-universal energy donor

-endergonic (energy released from breakdown of food substrate is used to create ATP)

-exergonic (energy is released from breakdown of ATP formed in endergonic reactions)

-consists of adenine, ribose, and 3 linked phosphates
Term
ATP formation
Definition
ADP + Pi --> ATP
Term
ATP breakdown
Definition
ATP --> ADP +Pi + energy via ATPase
Term
ATP breakdown/resynthesis
Definition
ATP <--> ADP + Pi + energy
Term
ATP <--> ADP + Pi + energy

energy = other pathways
Definition
-it's impossible to store enough ATP to meet energetic demands

-ATP is constantly broken down and resynthesized via 3 metabolic pathways

-pathways are EXERGONIC and provide the energy required to resynthesize ATP
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