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13 The Cytoskeleton
Powerpoint lecture
21
Biology
Undergraduate 1
03/06/2011

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Term
Immunofluorescence 
Definition
allows visualization of a specific protein or antigen
Term
antigen
Definition
a substance/molecule/enzyme/toxin that stimulates an immune response in the body (particularly production of antibodies)
Term
The cytoskeleton
Definition
a network of fibers that organizes structures and activities of the cell
Term
Roles of the Cytoskeleton (3)
Definition

Mechanical support

Motility

Regulation

Term
What are the three main types of fibers that make up the cytoskeleton?
Definition

Microtubules

Microfilamnets

Intermediate filaments

Term
Microtubules 
Definition

Thickest of the components of the cytoskeleton. 

Tubulin protein subunits


Functions - Maintenance of cell shape

-Cell motility

-chromosome movements in cell division

-Organelle movement

Term
Microfilaments
Definition

Two intertwined strands of actin, the thinnest components

Protein Subunits: actin

Main functions: -Maintenance of cell shape

-Changes in cell shape

-Muscle contraction

-Cytoplasmic streaming

-Cell motility

-Cell division

Term
Intermediate filaments
Definition

fibers with diameters in a middle range

proteins: one of several different proteins from the keratin family (fibrous)

-Maintenance of cell shape

Anchorage of nucleus and other organelles

Formation of nuclear lamina

-Stay on one place

Term
What is the most permanent cytoskeleton fixture?
Definition
Intermediate filaments
Term
What do intermediate filaments provide protection against?
Definition
mechanical stress
Term
What 3 classes of intermediate filaments are found in the cytoplasm?
Definition

Keratin filaments (in epithelial cells)

Vimentin-related filaments (in connective tissue, muscle cells, and glial cells)

Neurofilaments (in nerve cells)

 

Term
What class of intermediate filaments is found in the cell nucleus?
Definition
nuclear lamins (found all animal cells)
Term
What are the three possible organizing centers a microtubule could extend from?
Definition

A centrosome of a cell in interphase

A spindle pole of a mitotic spindle of a cell that is dividing

A basal body of a cilium in a ciliated cell (permanent, on eucaryotic cells)

Term
Which end of the microtubule is plus end and which is minus end?
Definition

the plus end: beta-tubulin end

the minus end: alpha-tubulin

Term
Microtubules are built from what type of molecule? What are those molecules made of?
Definition
molecules of tubulin which are dimers composed to alpha-tubulin and beta-tubulin bound together with non-covalent bonds
Term
In animal cells centrosomes contain a pair of ____. These structures are very similar to ___.
Definition

centrioles! (each with nine triplets of microtubules arranged in a ring)

 

basal bodies 

Term
The selective polarization of a cell can cause what?
Definition
polarization of a cell
Term
In nerve cells, all microtubules in the axon point in which direction?
Definition
all in the same direction, with their plus ends toward the axon terminal
Term
Motor proteins
Definition
use energy from repeated cycles of ATP hydrolysis  to travel along the actin filament or microtubule in one direction
Term
When the synaptic terminal of the motor neuron releases the neurotransmitter acetylcholine, what happens?
Definition
it depolarizes the muscle which causes it to produce an action potential
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