Term
| Is pseudomonas oxidase positive or negative? |
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Definition
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|
Term
| Where is pseudomonas found? |
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Definition
|
|
Term
| What is the metabolism of pseudomonas? |
|
Definition
| obligate aerobe but metabolically very versatile |
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Term
| How can you tell if it's psuedomonas grossly? |
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Definition
| white colonies that glow under black light and have a distinctive fruity smell |
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Term
| What diseases can p. aeruginosa cause? |
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Definition
| respiratory tract infections, endocarditis, bacteremia, bone and joint infections, CNS infections, ear infections, eye infections, skin and soft tissue, UTIs, GI infection, |
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Term
| A common, mild form of P. aeruginosa skin infection characterized by maculopapular rash= |
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Definition
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Term
| What are the main types of P aeruginosa virulence factors? |
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Definition
| proteases, ADP ribosyl transferases, pili, phospholipase C, cytotoxin (leukocidin), type III secreted toxins, rhamnolipids, alginate |
|
|
Term
| Name P. aeruginos protesases. |
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Definition
| alkaline protease, elastase, protease IV (at least 4 proteases) |
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|
Term
| Name the ADP-ribosyl transferases. |
|
Definition
| exotoxin A and exoenzyme S |
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|
Term
| What are the type III secreted toxins? |
|
Definition
|
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Term
|
Definition
| detergent-like glycolipids thought to be important for lung infections |
|
|
Term
|
Definition
| kills alveolar macrophages, inhibits cilia, inhibits phagocytosis |
|
|
Term
| What is an important virulence factor in CF lungs? |
|
Definition
| alginate because it inhibits phagocytosis and prevents antibiotics from reaching bacteria |
|
|
Term
| Which antibiotics are used for psuedomonas, burkholderia pseudomallei, or Burkholderia cepacia treatment? |
|
Definition
| extended spectrum beta lactams + aminoglycosides, flouroquinolones, colistin |
|
|
Term
| Where is burkholderia pseudomallei found? |
|
Definition
| environment in southeast asia and northern australia |
|
|
Term
| What organism appears as "onion skin" on BAP? |
|
Definition
| burkholderia pseudomallei |
|
|
Term
| How is burkholderia pseudomallei transmitted? |
|
Definition
| inhalation of dust or contact with dirt or dirty water (rice paddies) with abraded skin |
|
|
Term
| What are the four different courses whitmore's disease or melioidosis can take? |
|
Definition
| acute localized infection, acute bloodstream infection, pulmonary infection, or chronic supporative infection |
|
|
Term
| Melioidosis is also known as... |
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Definition
|
|
Term
| What allows B. psudomallei to live intracellularly? |
|
Definition
| effector proteins facilitate uptake of organism, help it to escape endocytic vesicles and form actin propulsion tails. formation of multinucleated giant cells also mediated by these proteins |
|
|
Term
| What allows burkholderia psuedomallei to have longevity in host? |
|
Definition
| intracellular living and/or in biofilms |
|
|
Term
| Where is B. ceptacia found? |
|
Definition
| environmental organism in soil and water |
|
|
Term
| Which species name talked about in this lecture actually represents a complex of different species? |
|
Definition
|
|
Term
|
Definition
|
|
Term
| How is B. cepacia spread? |
|
Definition
| person to person or through contaminated medications or medical devices |
|
|
Term
| What are the virulence factors of B cepacia? |
|
Definition
| quorum sensing; produces a potent protease |
|
|
Term
| What are the virulence factors of aeromonas hydrophila? |
|
Definition
| serine and metalloproteinases, multifunctional enterotoxin (hemolytic cytolytic, enterolytic) |
|
|
Term
| Where is aeromonas hydrophila found? |
|
Definition
| fresh and brackish waters, normally a pthogen of fish and amphibians |
|
|
Term
| Which disease discussed today is associated with fish and seafood? |
|
Definition
|
|
Term
| Aeromonas hydrophila is like what other bacteria in many ways? |
|
Definition
|
|
Term
| Diseases caused by aeromonas hydrophila in immune suppressed? |
|
Definition
| severe bacteremia, wound infection, cellulitis |
|
|
Term
| Aeromonas: oxidase? gram? shape? features? |
|
Definition
| oxidase positive, GNR, with a polar flagellum |
|
|
Term
| HOw do you treat aeromonas hydrophila diarrhea? |
|
Definition
|
|
Term
| How do you treat nondiarrheal infections with aeromonas hydrophila? |
|
Definition
|
|
Term
| shape/stain of stentotrophomonas maltophilia= |
|
Definition
|
|
Term
| Found where in environment? |
|
Definition
| isolated from ice machines and contaminated medical equipment |
|
|
Term
| Appearance of acinetobacter baumanii= |
|
Definition
| gram negative plump coccobacilli |
|
|
Term
| Appearance of acinetobacter Iwoffi? |
|
Definition
| gram negative plump coccobacilli |
|
|
Term
| Where are A. baumanii and Iwoffi found? |
|
Definition
| everywhere in the environment; could be considered normal flora |
|
|
Term
| Who are at risk of A. baumanii and A. Iwoffi infection? |
|
Definition
| those on broad spectrum antibiotics, recovering from surgery, or on mechanical ventilation |
|
|
Term
| How does exotoxin A work? |
|
Definition
| just like diphtheria toxin (ADPribosylates EF2 shutting down protein synthesis) |
|
|
Term
| How does exoezyme S work? |
|
Definition
| ADP ribosylates vimentin and GTP binding proteins; critical in burn wound infections |
|
|
Term
| How is phospholipase C virulent? |
|
Definition
| degrades surfactant and liberates inflammatory substances from AA metabolism |
|
|
Term
|
Definition
| ExoU is an acute cytotoxin while ExoY is an adenyl cyclase |
|
|
Term
| Ashdown's media is used for... |
|
Definition
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