Term
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Definition
| the amount of detail that can be seen in and around an object |
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Term
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Definition
| how distinct an object is from the background |
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Term
| Explain how resolution and contrast can be altered to improve the quality of an image. |
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Definition
| Resolution and contrast can be altered to improve image quality via microscope type and staining. |
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Term
| Function of bright field microscope |
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Definition
| light passes through specimen |
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Term
| Function of dark field microscope |
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Definition
| illuminates specimen from the sides (via reflected light) |
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Term
| Function of phase contrast microscope |
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Definition
| enhances contrast through phase-shifting of refracted light |
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Term
| Function of differential interference contrast microscope (DIC or Nomarski) |
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Definition
| uses 2 beams of polarized 90o polarized light to detect 3-D details in specimen |
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Term
| Function of fluorescence microscope |
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Definition
| uses excitation wavelength as illuminating light |
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Term
| Function of confocal scanning laser microscope |
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Definition
| computer builds up 3-D object with an aperture is used to shut out light from all but one focal plane of an object |
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Term
| Function of transmission electron microscope |
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Definition
| an electron beam passes through the specimen (using x-ray machine and magnet) |
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Term
| Function of scanning electron microscope |
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Definition
| detects electrons and x-rays scattered from a surface |
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Term
| Function of electron cryotomography (CryoEm) microscope |
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Definition
| "CAT scan for microbes" = microbe moves around the microscope |
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Term
| Function of atomic force microscope |
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Definition
| "feels" specimen by monitoring electric field |
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Term
| What happens in a simple stain |
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Definition
| single (+) dye attaches to (-) phospholipid head groups |
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Term
| What happens in a differential stain |
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Definition
| employs multiple stains to identify specimens based on bacterial properties. |
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Term
| What happens in a negative stain |
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Definition
| use of stain to make the background very dark against the specimen. |
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Term
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Definition
| to find information on cell shape, size, and cell arrangement |
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Term
| Why use a differential stain |
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Definition
| identify bacterial properties of a specimen/differentiate specimens w/in a sample |
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Term
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Definition
| to observe bacteria that are difficult to stain or fragile, or to observe cellular morphology since bacteria is not heat-fixed. |
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Term
| Uses for bright field microscope |
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Definition
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Term
| Uses for dark field microscope |
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Definition
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Term
| Uses for phase contrast microscope |
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Definition
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Term
| Uses for differential interference contrast microscope (DIC or Nomarski) |
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Definition
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Term
| Uses for fluorescence microscope |
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Definition
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Term
| Uses for confocal scanning laser microscope |
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Definition
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Term
| Uses for transmission electron microscope |
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Definition
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Term
| Uses for scanning electron microscope |
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Definition
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Term
| Uses for electron cryotomography (CryoEm) microscope |
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Definition
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Term
| Function of atomic force microscope |
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Definition
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