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VT 260 Week 2
Week 2
66
Veterinary Medicine
Undergraduate 4
04/15/2015

Additional Veterinary Medicine Flashcards

 


 

Cards

Term
ASAP. No longer than 15 minutes
Definition
How quickly do we need to get the agar in the incubator after antibiotic disks have been added?
Term
3% Hydrogen Peroxide
Definition
When doing a catalase test what do you need to add to your specimen?
Term
Bubbles=staph positive
No bubbles= staph negative
Definition
A positive catalase test will show what?
Term
Potassium Hydroxide
Definition
If you perform a KOH test, what is the additive?
Term
1% Tetramethyl-p-phenylenediamine
Definition
What is the reagent used in the oxidase test?
Term
True
Definition
On the MIC zone on your susceptibility test, the larger the area the lower the concentration of drug needed. True or False?
Term
EDTA preferred.
Definition
What is the reagent needed for the coagulase test?
Term
37 degrees C, 98.6 degrees F.
Definition
What should the temperature in the incubator be for normal culturing?
Term
Trace amount
Definition
When scoring a CMT,(California Mastitis test) what does the symbol T stand for?
Term
10-15 seconds
Definition
For a CMT, at what point do you score an initial test?
Term
To get a sample that represents the type of bacteria.
Definition
What is the objective of specimen collection?
Term
Normal flora
Definition
We have a draining tract, what are they frequently contaminated with?
Term
Pink
Definition
Gram negative bacteria will stain what color?
Term
Purple
Definition
Gram positive bacteria will stain what color?
Term
Enterobacteria. (Lactose fermenting Enterobacteriaceae)
Definition
You have a McConkey agar, it is selective media. It grows bacteria in the presence of bile salts and crystal violet. What bacteria is this good for?
Term
Solid media, or agar plates.
Definition
What culture media is the best and the most common used?
Term
To keep the condensation off the agar.
Definition
Why do we invert the agar plates when we put them in the incubator?
Term
Blood agar
Definition
What is the primary isolation media?
Term
Anaerobic bacteria
Definition
Your enrichment broth is used to grow what?
Term
Staphylococcus
Definition
You pull out a blood agar and have delta hemolysis. This is commonly produced by what bacteria?
Term
Glucose fermentation and the production of hydrogen sulfide.
Definition
The triple sugar iron agar is used for detection of what?
Term
Putting a candle in the jar and closing the lid tight. This will produce carbon dioxide.
Definition
How do you create an oxygen free environment?
Term
Gram negative
Definition
You have a McConkey agar, you see a healthy profuse growth. This is presumptive evidence of what organism?
Term
Pink
Definition
Urea tubes turn what color when they are positive?
Term
Anaerobic bacteria
Definition
Before you use a blood culture, you would use the thioglycollate broth to grow what?
Term
10-15 mls
Definition
How many mls are needed for a blood culture?
Term
Where did the specimen come from, what area?
Definition
When making the preliminary evaluation of primary cultures, what must you keep in mind?
Term
Hospital setting
Definition
Nosocromial infections come from where?
Term
Color outside the stab line. Everything is mixed.
Definition
Ho do you know if a motility test is positive?
Term
Isolating
Definition
What is it called when you are identifying microbes?
Term
Zoonotic diseases
Definition
What type of organisms do we not isolate?
Term
False because they need a host cell to survive.
Definition
All viruses are free living. True or False and why?
Term
Cocci are in pairs.
Definition
If an organism is diplicoccus, what does this mean?
Term
Binary fission
Definition
How do bacteria reproduce or replicate?
Term
Alpha hemolysis
Definition
On a blood agar, you see green growth, what type of hemolysis is this?
Term
Beta hemolysis
Definition
Complete hemolysis is termed what?
Term
Gamma hemolysis
Definition
No hemolysis is termed what?
Term
Gram staining
Definition
If you use crystal violet, iodine, and saphrine, what is the name of the staining?
Term
6.5-7.5 pH
Definition
Bacteria prefer what pH range?
Term
Insulated walls
Strong seal
Holds plates, tubes & jars
Adjustable thermostat
-35-37 degrees F for most bacteria
-25-30 degrees F for fungi
Humidity
-70-80%
Thermometer
Definition
What must a quality incubator have inside of it?
Term
Blood culture
Definition
Brucillis canins needs what type of culture?
Term
Psuedomonas
Definition
Gram negative bacilli, aerobic and is oxidase positive. What bacteria is this?
Term
Staphylococcus aureus
Definition
You have a gram negative cocci, that is catalase positive, and coagulase posiutive, what bacteria is this?
Term
Bacillus anthracis
Definition
You have a gram positive bacilli, it has spores but no hemolysis. What bacteria is this?
Term
National Committee for Clinical Laboratory Standards
Definition
What does NCCLS stand for?
Term
Central, subterminal & terminal
Definition
What are the three categories of spores?
Term
Crustal Violet (30-60 seconds)
Rinse
Iodine (30-60 seconds)
Rinse
Decolorizing clear (10-15 seconds)
Rinse
Saphrine( 30-60 seconds)
Rinse
Definition
List the gram stain in order:
Term
To grow individual colonies of specimen.
Definition
What is the goal for streaking plates?
Term
psychophiles
Definition
What bacteria grow best at low temperatures?
Term
Thermophilies
Definition
What are bacteria called that like to grow at high temperatures?
Term
Punctiform
Definition
When describing the size, what are tiny colonies reffered to as?
Term
Elevation
Definition
This will describe the side view of a colony.
Term
Flat
Raised
Umbonmate
Crateriform
Convex
Pulvinate
Definition
These are the most common types of elevation:
Term
Blood agar
Definition
Most widley used inoculation media, will grow most bacteria positive and negative. Standard media selected to help identify colony morphology and hemolytic patterns.
Term
MacConkey agar
Definition
This inoculation media provides environment similair to intestines. Selective for bacteria that grow in presence of bile salts. Gram negative only.
Term
Enrichment broth
Definition
Least selective inoculation media. Must combine with microscopic evaluation.
Term
Mueller-Hinton agar
Definition
This inoculation media is an exclusion test that does not allow growth of streptococcus.
Term
Bacillus Cereus
Definition
A gram positive Bacilli with spores that is hemolytic is what bacteria?
Term
Listeria
Definition
A gram positive Bacilli that has no spores, is catalase positive, and motile is what bacteria?
Term
Corynebacterium
Definition
A gram positive Bacilli that has no spores, catalase positive, and non-motile is what bacteria?
Term
Arcanobacterium
Definition
A gram positive Bacilli that has no spores, is catalse negative with alpha hemolysis is what bacteria?
Term
Erysipelothrix
Definition
A gram positive Bacilli, that is catalse negative with beta hemolysis is what bacteria?
Term
E-coli
Definition
A gram negative Bacilli that is aerobic and oxidase negative is what bacteria?
Term
Bacteroides
Definition
A gram negative Bacilli that is anaerobic is what bacteria?
Term
Staphylococcus epidermidis
Definition
A gram positive cocci that is catalase positive, and coagulase negative is what bacteria?
Term
Streptococcus spp.
Definition
A gram positive cocci that is catalase negative is what bacteria?
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