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MOLECULAR BOR
MOLECULAR BOR
22
Chemistry
Undergraduate 4
11/20/2011

Additional Chemistry Flashcards

 


 

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Term

 

Which nucleotide pair has high frequency of mutation in human chromozomal DNA?

Definition

CG

Spontaneous deaminazation of methylcytosine results in thymine formation; it occurs 2.5 X more frequently than any other single nucleotide mutation

Term
During the 2 phase of the cell cycle, the DNA content of a cell is
Definition

Tetraploid

 

The G2 phase of the cell cycle follows the S phase, where NA content goes from 2N to 4N or from diploid to tetraploid

Term
Intervening -sequences are found in
Definition

Hetero-nuclear RNA

Hetero-nuclear RNA is made up of newly transcribed mRNA that still contains intervening sequences.

Term
Which is considered an epigenitic modification of DNA
Definition

Methylazaton of CpG islands in the promoter region of a gene.

 

Methylazation of CpG islands is an epigenetic mechanism of gene silencing.

Term
A metaphase chromozome with primary constriction that gives the chromozome clearly defined short and long arms is considered
Definition

Sub-metacentric

 

Chromosomes with clearly defined short and long arms are known as submetricentric chromosomes

Term
Reverse transcriptase would best be described as a:
Definition

RNA dependent DNA polymerase

 

RT synthesizes a DNA molecule from an RNA template

Term

The 2 allels for a sex-linked resessive disease are X and x. The mutant alleal is x. What is the percentage of male offspring that would be expected to be affected by the disease from from parents who have the following genotypes? 

mother Xx

Father xY

 

Definition

50%

One X chromosome is passed on to all sons

by the mother; since she is a carrier of a mutant X alleal, 50% of her sons will be affected.

Term
The term that best describes males reguarding the X-linked genes is:
Definition

Hemizygous

 

Males are hemizygous for X linked allels because they have only one copy of the X chromozome.

Term
The mode of inheritance of mitochondrial DNA is:
Definition

Maternal

 

All mitochondra are passed on from a mother to her children, but not by a father to his children

Term
Which statement concerning the resoluton of DNA on agarose gel is true
Definition

As the concentration of agarose in the gel increases, the smaller the size of DNA that can be resolved on the gel.

 

The greater the concentration of agarose, the smaller the size of DNA that can be resolved.

Term
An RFLP is most likely detected using which of the following methods?
Definition

Southern blot

 

RFLP analysis is preformed on DNA. Southern blotting id the method used to separate DNA fragments bby size, transfer the DNA fragments to a solid support, and then hybridize with a probe specific for the gene of interest,

Term
The methodology of performing a reverse dot blot is described as:
Definition
Attaching multiple probes to a nitrocellulose membrane and then hybridizing a singlelabeled patient sample to multiple probes
Term
Which condition has the higest stringency for washing a southern blot after hybridization has been completed.
Definition
High temperature ,low salt concentration
Term
The Klenow fragment of E-coli DNA polymerasse 1 is used
Definition
To label DNA probes by random primer lableing
Term
What is a solution hybridization method for the detection of nucleic acid protein interaction?
Definition
gel mobility shift assay
Term
Which probe is most often used to detect trisomy 21 by interphase fish?
Definition
Alpha satelitte DNA probe
Term
How many volumes of ethanol are added to 1 volume of a DNA salt solution to cause the DNA to precipitate?
Definition
2
Term
Chelex 100 resin is used to:
Definition
Extract DNA from cells
Term
The fluorescent dye that exhibits the greatest sensitivity for quantitation of DNA is:
Definition
SYBR Green
Term
When quantifying the amount of genomic DNA in a sample by spectrophotometry, an OD 260 of 1.0 cooresponds to what concentration of DNA
Definition
50 ug/mL
Term
An RNA sample is isolated from pheriheral blood cells of a patient. When performing spectrophotometry analysis to determine the yeald of RNA in the sample you find the 1:40 dilution of the 0.5 mL sample gives an OD 260 reading of 0.03125 and an OD 280 reading of 0.01760. What is the total amount of RNA contained in the 0.5mL sample?
Definition
25 ug
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