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Microsscopy Techniques
N/A
46
Microbiology
Undergraduate 1
06/10/2013

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Cards

Term
Micrometer
Definition
10(-6)
Term
Nanometer
Definition
10 (-9)
Term
compound light microscope
Definition
has a series of lenses and utilizes visible light as source of illumination
Term
light microscopy
Definition
any kind of microscope that uses visible light
Term
illuminator
Definition
the light source
Term
condenser
Definition
directs light rays through specimen
Term
objsective lens
Definition
lens closest to object
Term
ocular lens
Definition
eye piece
Term
total magnification
Definition
objective lens magnification x ocular lens
Term
resolution
Definition
ability of lenses to distinguish fine detail
Term
refractive index
Definition
measure of the light bending ability of a medium
Term
brightfield illumination
Definition
by focusing light
Term
darkfield microscope
Definition
used for examining live microrganismsthat are invisible in natural light or can't be stained
Term
phase-contrast microscope
Definition
permits detailed examination of internal structures
Term
Differential interference contrast microscopy
Definition
2 beams of light to observe refractive indexes
Term
fluorescence microscopy
Definition
ability of substances to absorb short wavelengths of light and give off light at a longer wavelength
Term
antigen
Definition
foreign substances
Term
confocal microscopy
Definition
small region illuminated with a laser
Term
scanning acoustic microscopy
Definition
interprets the action of a sound wave through a specimen
Term
electron microscope
Definition
beam of electrons used instead of light
Term
transsmission electron microscope
Definition
directing beams of electrons to illuminate specimen
Term
scanning electron microscope
Definition
provides 3d views of specimans
Term
scanned probe microscopes
Definition
use of probes to observe cells
Term
scanning tunneling microscopy
Definition
uses metal probe that scans a specimen and produces an image revealing bumps and depressions of the atoms on the surface
Term
atomic force microscopy
Definition
metal and diamond probe forced down onto a specimen. as it moves it records movements into a 3d image
Term
staining
Definition
coloring microorganisms with a dye that emphasizes certain structures
Term
fixed
Definition
attached to slide
Term
smear
Definition
thin film of material attached to a slide
Term
basic dyes
Definition
positive ion
Term
acidic dyes
Definition
negative ion
Term
negative staining against
Definition
preparing a colorless bacteria against a colored background
Term
simple stain
Definition
aqueous stain or alcohol solution of a single basic dye
Term
mordant
Definition
a chemical added to a solution to intensify the stain
Term
differential stain
Definition
distinguish different types of bacteria
Term
gram stain
Definition
classifies bacteria
Term
primary stain
Definition
imparts color to all stains
Term
decolorizing agent
Definition
removes color from cells
Term
gram positive
Definition
keep color
Term
gram negative
Definition
loses color
Term
counterstains
Definition
have contrasting colors to primary stains
Term
acid-fast stain
Definition
binds to bacteria that have waxy material in cell walls
Term
special stains
Definition
used to color and isolate endospores and flagella
Term
capsule
Definition
microorganisms that have a gelataneous covering
Term
virulence
Definition
the degree to which a pathogen can cause a disease
Term
endospore
Definition
resistant, dormant structure formed within a cell that protects bacterium from adverse environmental conditions
Term
flagella
Definition
structures of locomotion too small to be seen with a light microscope without staining
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