Home > Flashcards > Biology > microscopy

Details Title: microscopy Description: dissecting and compound microscopes Total Flash Cards: 21 Created: 01/31/2005 16:07:43	To study from this set of flash cards, or to create your own your own study flash cards, register HERE. If you are already a registered user, CLICK HERE.
Cards Term dissecting microscope Definition useful in studying surface structures of 3d objects. 2 light sources: mounted above for opaque, below for transparent objects Term how a dissecting micro works Definition reflected light bounces off the sample, passes through an objective lens, and then through a par of ocular lenses to your eyes. magnification range of 10x-30x Term compound micro Definition uses a double lens system. magnifies up to 1000x Term ocular lens Definition found in eyepiece, usually 10x in power Term objective lens Definition mounted on a rotating disk. 4x, 10x, and 40x Term arm Definition supoorting body of scope Term eyepieces/oculars Definition house the ocular lenses. 10x Term body tube Definition hollow tube providing the right optical distance between th eocular and objective lenses Term revolving nosepiece Definition the mount for the objective lenses; revolves to bring the various objective lenses into position for viewing object Term objective lenses Definition lenses of various sizes and magnification powers. lowest lens has shortest tube, but provides largest viewing diameter Term stage Definition platform on which the microscope slice is placed under the objective lens Term substage lamp Definition incorporated into the base of the microscope Term substage condenser Definition a lens systme used to focus the light beam up into the iris, and the slide Term iris diaphragm Definition regulates light intensity. controls amount of light transmitted through the slide Term coarse focus adjustment knob Definition adjusts the coarse focusing. Term fine focus adjustment knob Definition adjusts the fine focus Term monocular scope Definition look into the ocular with the eye that is opposite of writing hand Term binocular scope Definition first adjust the width of the two eyepieces, until the distance matches your eyes. Term parfocal Definition once the proper focus is adjusted for one objective lens, the miage should remain sharp when switching to another objective lens Term preparing a wet mount Definition 1) place 1-2 drops of water on a slide 2) place coverslip over specimen slowly so there are no air bubbles 3) first examine under low power 4) go to a higher power to see specific objects Term depth of field Definition focusing on one part of an object causes the others to go out of focus, the lower the magnification, the greater the depth of field