Shared Flashcard Set

Details

Microbiology lab Midterm
SHU Micro lab midterm
18
Microbiology
Undergraduate 2
02/28/2010

Additional Microbiology Flashcards

 


 

Cards

Term
What is the shape of Ecoli
Definition
Rods
Term
Microscope that shows dark objects in a brighter field
Definition
A brightfield compound microscope
Term
What are the basic parts of a microscope?
Definition

  • base
  • stage
  • arm
  • body tube
  • condenser
  • iris diaphragm
  • objective lenses
  • ocular lens
  • coarse adjustment knob
  • fine adjustment knob

Term
What are the 4 possible objective lense on a normal compound microscope and what are their powers?
Definition

  1. 4 x - scanning power
  2. 10 x - low power
  3. 40 x - high power
  4. 100 x - immersion oil

Term
Refers to the ability of lenses to reveal fine detail or 2 points distinctly separated
Definition
resolution or resolving power
Term
A magnifying lense with 10 x power and part of the eyepiece
Definition
ocular lens
Term
The total magnification obtained when the 4x (scanning) lens is in place is?
Definition
40 x
Term
The total magnification obtained when the 10 x (low power) lens is in place ?
Definition
100 x
Term
A culture that only contains one kind of microorganism
Definition
pure culture
Term
A differential stain to be use to differentiate between microorganisms
Definition
gram staining
Term
What are the 4 Gram staining regents used?
Definition

  1. Crystal violet
  2. Iodine
  3. Decolorizing agent
  4. safran

Term
What color appears on a gram negative organism ?
Definition

clear to pink

Term

What color does a gram positive organism appears

Definition
purple
Term
Why is agar preferable to gelatin as a solidifying agent in culture media?
Definition
agar has no nutritional value so bacteria cannot feed off of it. The agar remains solid until it is melted at 100 degrees celcius and then cooled at 40 degrees celcius where it remains solid. Solid agar is better for bacteria to grow because microbes can't degrade it.
Term
What can enhance resolving power?
Definition
1) To shorten the distance from the objective lens and the ocular lense. Smaller wavelengths of light improve resolving power.
Term
The main functions of a microscope 
Definition
Resolution and Magnification of a compound microscope
Term
limit of resolution
Definition
They refer to the ability of a microscope to distinguish between two objects.
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