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mEDIA portion of quiz
quiz one lab
31
Microbiology
Undergraduate 2
03/16/2011

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Term
Media
Definition
Chemicals used for the matinence of life, growth and reproduction of microbes.
Term
In vitro Media
Definition
Media which only contains inanimate materials. Used for Bacteria and Fungi.
Term
Bacteria and Fungi use what kind of media?
Definition
In Vitro Media
Term
In Vivo Media
Definition
Media which living cells (chick embryo,live animal or tissue culture use to live) Used for viruses and Ricikettsia.
Term
What media type is used for Viruses and Ricikettsia?
Definition
In Vivo Media.
Term
What is the universal biological solvent in media?
Definition
H2O
Term
What nutrient compounds are used in all media?
Definition
C,O,N,S,H,P
Term
What are the inorganic nutrients?
Definition
Co2 H2S,NH3 , Phosphates, and sulfates
Term
What are the organic nutrients?
Definition
Sugars, Fatty Acids,Amino Acids, Nucleic Acids.
Term
Growth Factors-
Definition
Essential chemicals which cannot be made by bacteria but are critical to metabolism.
Term
Nutrients
Definition
Compounds containing essential elements (C,O,N,S,H,P) which are oxidized to generate energy (ATP) or reduces to build cellular structures.
Term
Inorganic G-F Growth Factors
Definition
Ca+,Mg++,Si++,Fe++,Co,Na+,K+
Term
Organic G-F Growth Factors
Definition
Vitamins and certain Amino Acids.
Term
Bacteria Require a ________pH.
Definition
Neutral
Term
Fungi prefer an _________pH.
Definition
Acidic
Term
pH of Bacteria Media
Definition
6.8-7.2
Term
pH of Fungal Media
Definition
5.5-6
Term
Synthetic Media
Definition
Media in which the exact chemical formulation is known.
Term
Non-Synthetic Media
Definition
not all chemicals are known(blood, tissues etc.)
Term
Use of media
Definition
purposes of various ingridents to promote growth
Term
general Purpose media
Definition
allows a large number of bacteria to grow.
Term
Differential Media
Definition
Colony Morphology on agar gives clues to ID
Term
Selecive Media
Definition
Only Allows certain bacteria or fungi to grow ( dyes,pH)
Term
Transport Media
Definition
Temporary holding media-preserves bacteria for lab.
Term
Agar melts at
Definition
95C
Term
agar can be poured at
Definition
50C
Term
agar solidifies at
Definition
42C
Term
Ingredients of Nutrient Broth and Nutrient Agar
Definition
1. H2O as biological solvent
2. peptone-protein digest which provides nutrients
3. Beef extract:provides groth factors
4. NaCl:to adjust Osmotic Pressure
5. Agar as solidifying agent.
Term
Enriching Media
Definition
Additives:Serum,yeast extract,blood,liver...etc
Term
Indicators used in Media
Definition
Indicators/dyes used to ID the pH and changes to pH as bacteria metabolize specific media(bromthymol Blue, Phenol Red, Methyl Red.)
Term
Forms of Media Used in Micro 33
Definition
1. broths
2. Slants
3. Plates
4. Fermentation Tubes
5. FTM-Thioglycollate Broth (O2 requirements)
6. stabs
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