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Lecture 38
Brock
9
Microbiology
Undergraduate 3
04/24/2014

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Term
transfection
Definition
describes the introduction of DNA not mammalian cells even though no viral nucleic acid is involved
Term
adding DNA with cationic carriers that bind to and protect DNA;
allowing DNA to be taken up by phagocytosis;
DNA injected directly into the nucleus
Definition
How is transfection in mammalian cells accomplished?
Term
shuttle vectors
Definition
vectors that can replicated and be stably maintained in 2 or more unrelated hosts

allows for movement of genes between unrelated organisms without modification of the vector
Term
expression vector
Definition
allows for control of expression of clones gene, contains regulatory sequences for manipulation of expression
Term
integrating vectors
Definition
expression vector developed to be stably maintained in host, maintained at low copy number
Term
bacteriophage M13
Definition
infectious virus vector that replicates without killing its host
Term
cosmids
Definition
plasmid vectors with the cos site from the lambda genome

can be packaged into lambda virions, more stable than plasmids,
Term
bacterial artificial chromosomes (BAC)
Definition
vector based on F plasmid
Term
Yeast artificial chromosomes (YACs)
Definition
hold most DNA of any vector but has problems with rearrangement and recombination
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