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Inhibiting Microbial Growth In Vitro
Test Three
30
Microbiology
Undergraduate 3
02/14/2013

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Cards

Term
Steralization
Definition
complete destruction
Term
Disinfection
Definition
decrease the amount of pathogens
Term
pasteurization
Definition
heats fluid to kill pathogens but not bacteria
Term
Disinfectant
Definition
innanimate objects: too harsh for skin
Term
antiseptic
Definition
disinfecton for skin: pathogens
Term
sanitization
Definition
knocking out all kinds of growth
Term
What do microbial agents kill?
Definition
microbes
Term
What do germicidal agens kill?
Definition
pathogens
Term
Microbistatic agents
Definition
wont kill but will prevent from growing
Term
Bacteriostatic agent
Definition
keeps bacteria from growing
Term
Sepsis
Definition
no notes
Term
Asepsis
Definition
prevents infection from starting
Term
Time and temperature have an _____ relationship
Definition
inverse
Term
Thermal death point
Definition
point at which 99% will be killed off
Term
thermal death time
Definition
pick a temperature: measures how long it takes for them to die
Term
Where is dry heating useful
Definition
when using metals and glassware
Term
What types of dry heating is available?
Definition
baking, incineration and flaming
Term
What is the faster, more effective way to heat kill things
Definition
moist heat: boiling or steaming (lower temps, less destruction)
Term
Autoclaving
Definition
moist heat and pressure
Term
What kills bacterial endospores, can be used on liquids or solids and has wet and dry cycles?
Definition
autoclaving
Term
What preserves bacteria?
Definition
cold; inhibits growth by slowing metabolic activity (fast freezing)
Term
desiccation
Definition
drying or freeze drying
Term
What are the two main methods to inhibit growth?
Definition
radiation and filtration
Term
What are the types of radiation?
Definition
UV, X rays, gamma rays, beta rays
Term
What are the types of filtration?
Definition
membrane filters, HEPA filters, Class 2 biological safety cabinets, critical air filtration
Term
What is a ohysical method to inhibit growth?
Definition
give bacteria an environment that they cannot grow in
Term
What ways can you turn the atmosphere gaseous?
Definition
ethylene oxide, remove the oxygen, or add oxygen
Term
Using disinfectants: what are the factors to consider? 8
Definition
prior cleaning, organic load, bioburden, concentration of disinfectant, contact time, topography of object being disinfected, temperature/pH, organisms to be killed
Term
Using disinfectants: the ideal chemical antimicrobial agent
Definition
broad antimicrobial spectrum, fast acting, not to be affected by organic matter, not harmful to tissue or materials, should leave residual, water soluble, inexpensive, easy to prepare, simple directions, stable as a concentrate and working solution, odorless
Term
What are some of the pathogens in food
Definition
salmonella, campylobacter, E coli, cyclospora
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