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Final Practical
Final Lab Practical
45
Microbiology
Undergraduate 2
12/03/2013

Additional Microbiology Flashcards

 


 

Cards

Term
Selective Media
Definition

Culture media allows some organisms to grow, but inhibits the growth of other organsims are called______media.

Term
Differential media
Definition
Culture media that supports the growth of many organisms, but allows observation of visible differences based on differing characteristics among the various organisms is called_______media.
Term
High Salt Concentration
Definition
The inhibitor used in mannitol salt agar is___?
Term
Gram negative organisms
Definition
MacConkey agar is selective for the growth of ________organisms.
Term
Staphylococcal species
Definition

Mannitol Salt agar is selective for the growth of _________organisms.

 

Term
Gram negative organisms
Definition
Eosin Methylene Blue (EMB) agar is selective for the growth of ________organisms?
Term
fungi
Definition
Sabourad Dextrose agar (SDA) is selective for the growth of _________organisms?
Term
Phenol Red
Definition
The pH indicator used in mannitol salt agar is _______?
Term
Lactose
Definition
Growth of pink or purple colonies on MacConkey agar is indicative of the fermentation of_________?
Term
Lactose
Definition
Eosin Methylene Blue agar is differential based on the fermentation of____?
Term
Yellow
Definition
Mannitol fermentation produces acid which lowers the pH and causes the phenol red indicator to turn_____?
Term
Lactose
Definition
Growth of dark pink or purple colonies on Eosin Methylene Blue agar is indicative of the fermentation of_____?
Term
Dark pink or purple
Definition
Lactose fermentation is indicated on Eosin Methylene Blue agar by the growth of _______colored colonies?
Term
Pink or red
Definition
In the absence of mannitol fermentation, the indicator remains what color?
Term
pale pink, white, or colorless
Definition
Non-lactose fermenters appear what color on both MacConkey and EMB agar?
Term
E COLI
Definition

Which of the following bacteria would grow in MacConkey agar?

E Coli

S auerus

S epidermidis

Streptococcus pneumoniae

Term
Staphylococcus auerus
Definition

Which of the following bacteria would grow in Mannitol Salt agar?

Streptococcus pneumoniae

S aureus

E coli

Pseudomonas aeruginosa

 

Term
E coli
Definition

Which of the following organisms would grow in EMB agar?

S auerus

Strep pneumo

Enterococcus fecalis

E coli

Term
Staph auerus
Definition

Which of the following organisms seen in lab fermented mannitol on the mannitol salt agar?

S auerus

Staph epidermidis

Strep pneumo

E coli

 

Term
E coli
Definition

Which of the following seen in lab fermented lactose in the Macconkey agar by turning the colonies pink?

Pseudomonas aeruginosa

Strep pneumo

Staph auerus

E coli

Term
E coli
Definition
Which of the following bacteria seen in lab fermented lactose on the EMB agar by turning the colonies dark purple?
Term
Catalase
Definition
What enzymes turns hydrogen peroxide into oxygen and water?
Term
Oxidase
Definition
What enzyme turns the bacteria purple on the filter or white paper?
Term
Pseudomonas aueriginosa
Definition

Which of the following organisms is catalase positive?

Strep pneumo

Entercoccus fecalis

E coli

Stap aureus

Pseudo aeruginosa

Term
Pseudomonas aeruginosa
Definition

Which of the following bacteria is oxidase positive?

E coli

Pseudo aeruginosa

Staph auerus

Strep pneumo

Entercoccus fecalis

Term
Catalase
Definition
What enzyme is used to differentiate between Staph and Strep?
Term
Staphylococcus
Definition
Which species of gram positive organisms is known to be catalase positive?
Term
Streptococcus
Definition
Which species of gram positive organims is known to be catalase negative?
Term
Staphylococcus
Definition
Which species of gram positive bacteria has a morphology of cocci in clusters?
Term
Streptococcus
Definition
Which species of gram + bacteria has a morphology of cocci in chains?
Term
Streptococcus pneumoniae
Definition
Which strep which you identified in lab has a gram positive diplococci appearance and shows as alpha hemolysis on blood agar?
Term
Enterococcus fecalis
Definition
Which streptococcus has a gram + cocci in chains appearance and shows as gamma hemolysis on blood agar?
Term
Staph aureus
Definition
Which Staph species is the only species that is coagulase+?
Term
Crystal Violet-Iodine-Alchohol decolorizer-Safranin
Definition
What are the order of solvents used in gram staining?
Term
Gram Stain
Definition

Which of the following did you have to do first when you tried to identify your unknown organism?

Grow the organism on mannitol salt agar

Grow the organism on macconkey agar

Catalase test

Oxidase test

Gram stain

Term
Catalase
Definition
After gram staining your bacteria, it shows a gram positive cocci and you are unclear whether it is Strep or Staph based on morphology alone, which test would you run to differentiate between the two?
Term
Either macconkey agar or EMB agar
Definition
After gram staining your bacteria it shows to have the morphology of a gram negative bacilli, and now you want to differentiate between whether it ferments lactose or not, which agar would you use?
Term
Mannitol Salt agar
Definition
After gram staining your bacteria, it shows to have a gram+ cocci in cluster morphology, which agar would you use to differentiate between the species of this organism?
Term
Pseudomonas aeruginosa
Definition
Which organism did you identify in lab which had a gram- bacilli morphology, non lactose fermenter, and was oxidase positive?
Term
E coli
Definition
Which organism did you identify in lab which showed a gram- bacilli morphology, fermented lactose and was oxidase negative?
Term
Salmonella typhi
Definition
Which organism did you identify in lab whic showed a gram- bacilli morphology, non lactose fermenter and was oxidase negative?
Term
Staph aureus
Definition
Which organism did you identify in lab that showed a gram positive cocci in clusters, was catalse postive, and fermented mannitol?
Term
Entercoccus fecalis
Definition
Which organism did you identify in lab that showed a gram positive cocci in chains morphology, was catalase- and showed gamma hemolysis on blood agar?
Term
Green
Definition
What color does Pseudomonas aeruginosa turn on nutrient agar which you witnessed in lab?
Term
Trypticase soy agar
Definition
What agar was used to show hemolysis in the Streptococcus species in lab?
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