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Evacuated Tubes
All about evacuated tubes
40
Biology
Professional
09/10/2013

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Term
Blood Culture Bottles: Additives
Definition
Culture Broth and Agar Medium
Term
Blood Culture Bottles: Two Sets
Definition
Aerobic and Anaerobic
Term
Blood Culture Bottles: Purpose
Definition
help determine the presence and extent of infection, indicate type of organism responsible, and the antibiotic to which it is susceptible
Term
Blood Culture Bottles: Reason to Order
Definition
FUO(fever of unknown origin), bacteremia/ septicemia
Term
Blood Culture Bottles: Special Requirments
Definition
second sites and skin antiseptics (isopropyl alcohol)
Term
Blood Culture Bottle: Remember to...
Definition
Draw a min and max line; don't want to little blood; exhaust vacuum in tube
Term
Light Blue: Purpose
Definition
used for most coagulation/hemostasis testing; most common tests arePT (protime)and PTT (partial thromboplastin time); other tests include fibrinogen and factor assays
Term
Blood Culture: Order of Draw
Definition
1st
Term
Light Blue: Order of Draw
Definition
2nd
Term
Light Blue: Additive
Definition
sodium citrate
Term
sodium citrate
Definition
an anticoagulant that binds calcium. since calcium is needed for clot formation, when it is unavailable, the blood is anticoagulated
Term
Light Blue: Type of Tube
Definition
Anticoagulated
Term
Light Blue: After Centrifugation
Definition
cells stay in liquid forma and go to bottom and the plasma stays on top. Sample can be mixed up again and return to whole blood
Term
Light Blue: # of Inversion
Definition
3-4 times, immediately after drawing the tube for adequate mixing of blood and anitcoagulant
Term
Light Blue: Rember
Definition
MUST ALWAYS BE COMPLETELY FILLED because of the 9 to 1 blood to additive ratio
Term
Light Blue: Special Requirements
Definition
If drawing from a butterfly needle a waste tube (red with silica or another blue) is recommended
Term
Light Blue: Considerations
Definition
The tests performed on this tube can be picky and there may be specified time limits for delivery to lab. Centrifuge time and speed can also impact testing.
Term
Gold Topped Tube: Type
Definition
SST= Serum Separator Tube
Term
Greiner Tube
Definition
have a red top with a gold ring on it
Term
Gold Topped Tube: Tests
Definition
most common tube for all routine chemistry testing
Term
Gold Tube: Inversions
Definition
5 times immediately after drawing
Term
Gold Tube: Additive
Definition
Silica: Clot Enhancer
Term
Gold Tube: Category
Definition
Coagulated: Serum
Term
Gold Tube has a
Definition
separator gel so after centrifuging the cells are on bottom the gel in the middle and the serum on top; the cells are inaccessible
Term
Gold Tube: Special Requirments
Definition
must stand upright for 30 min after it is drawn and mixed so that the blood can clot before it is centrifuged
Term
Gold Tube: Disadvantage
Definition
NOT useful for stat chemistry tests because it can NOT be centrifuged immediently
Term
Gold Topped Tube: Identical Tube?
Definition
Marble or Tiger Topped
Term
Red Tube: Additive
Definition
Silica, a clot activator
Term
Red Tube: Inversion
Definition
5 times immediately after drawing
Term
Red Tube: Testing
Definition
Chemistry, Immunology/Serology, some Blood Banks
Term
Red Tube: Serum is identical to
Definition
Gold Topped Tube
Term
Red Tube: does not have
Definition
a separator gel
Term
Red Tube: Category
Definition
Coagulated; Serum
Term
Red Tube: Remeber
Definition
Serum may need to be removed to keep some chemistry values from changing
Term
Red Tube: Special Direction
Definition
tube must stand upright for 30 min before it can be centrifuged to allow the blood to clot
Term
Red Tube: Disadvantage
Definition
NOT useful in chemistry STAT testing because it can NOT be centrifuged immediantly
Term
Red Tube: Chemistry Testing EX
Definition
drug testing and specialized test
Term
Red Tube: Blood Bank Testing EX
Definition
types and screens, crossmatch, and RH
Term
Red Tube: Immunology/Serology Testing EX
Definition
a variety of tests
Term
Red Tube: It is possible to
Definition
knock red cell off of the clot for select testing and blood bank testing (there is no gel to form a barrier over the clotted cells)
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