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Electron Microscopy
cool
7
Biology
9th Grade
09/24/2013

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Term
an electron beam
Definition
has a wave length 100,000 times shorter than white light. thus, the resolution of an electron microscope is far superior to that acheived by a compound light microscope.
Term
EM
Definition
Transmission / Scanning
Term
Transmission
Definition
- Internal structures of cells and viruses.
- Requires an extremely thin section of specimen (100m)
- No 3.D image
Term
Scanning
Definition
-Surface structures of cells and viruses
-Whole cells are viewed
-3D image
Term
Disadvantage of EM over CLM
Definition
The specimen is dehydrated, stained with heavy metal such as lead or osium,and viewed under high vaccum. This may lead to the introduction of artifacts- structures not originally present in the specimen.
Term
(viruses, internal structures of cells), are smaller than 2um.
Definition
must be viewed under an electron microscope.
Term
(viruses, internal structures of cells), are smaller than 2um.
Definition
must be viewed under an electron microscope.
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