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Cytology
Study aid for cytology
10
Veterinary Medicine
Professional
11/16/2012

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Cards

Term
5 categories for classifying FNA cytology samples
Definition
Cystic
Hemorrhagic
Inflammatory
Mixed cell population (inflam atypical cells)
Neoplastic
Term
4 tissue of origin categories for FNAs of neoplasms
Definition
Epithelial
Mesenchymal
Round
Neuroendocrine (naked nuclei)
Term
When you have a cerebrospinal fluid (CSF) sample from a patient, WHO YA GONNA CALL?
Definition
CSF samples must be processed or preserved within 60 minutes of collection. During the daytime regular hours, just turn the sample in appropriately. If it is after 5:30 pm, the sample MUST BE submitted as a STAT request, and the ON-CALL CLIN PATH *RESIDENT* must be called (not the on-call clin path tech).
Term
When submitting a cerebrospinal fluid (CSF) sample, a critical piece of information MUST be included with the submission in addition to the other information normally submitted with any cytology sample - what is the critical information that must be included?
Definition
Anatomic site of CSF collection. Since there is caudodirectional flow of CSF, the NORMAL protein concentration is lower in samples collected from the atlanto-occipital (AO) site (also called cerebromedullary cistern (CMC)) compared to the lumbosacral (LS) site. We NEED!!! to know the site of collection in order to accurately interpret the protein concentration of the sample.
Term
When trying to diagnose POLYarthropathy (disease of multiple joints), synovial fluid should be collected from HOW MANY joints??
Definition
Autoimmune, nonerosive polyarthropathy is the most common inflammatory arthropathy of dogs. To accurately diagnose a POLYarthropathy, one must analyze synovial fluid from MORE THAN ONE joint. So, collect fluid from at least two joints! Sticklers would have you sample six joints. (we're not sticklers in these parts).
Term

Classify the lesion

 [image]

Definition

This is an inflammatory lesion that happens to show neutrophilic inflammation with bacterial infection. There are 5 main categories used to classify cytology aspirates:

  1. cystic, 
  2. hemorrhagic, 
  3. inflammatory, 
  4. mixed cell population, 
  5. and neoplastic.
Term
What critical bit of information should be included with cytology and histology submissions?
Definition
signalment and pertinent history: eg, location of mass, duration of mass, gross description of the mass/organ, current steroids or antibiotics, etc.
Term
When collecting cytology samples from an animal with multiple enlarged lymph nodes, which location should be avoided? ---we didn't cover this in 2013--- optional
Definition
Submandibular lymph nodes. Submandibular lymph nodes often have an increased number of lymphoblasts compared to other lymph nodes because of antigenic stimulation from the oral cavity. Occasionally, this can confound lymphoma diagnosis. If you're trying to dx lymphoma and there are multiple big nodes, sample other ones, like prescapular, popliteal.
Term
Name generalizations about benign neoplastic lesions
Definition
Cells are uniform with minimal size and shape variation of cells and nuclei. Nuclear chromatin is condensed.
Term
When aspirating a very enlarged lymph node, what is it a good idea to try to aim the needle toward the periphery of the node? ---we didn't cover this in 2013--- optional
Definition
The center of very enlarged lymph nodes may be necrotic. During FNA, redirect the needle toward the periphery of the node, to try to get cells from a non-necrotic portion of the node - increases the likelihood of a diagnostic sample. If just necrosis is collected, the cytologic diagnosis will be NECROSIS with little or no indication of the underlying etiology.
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