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Clinical Instrumentation - Nephelometry
Clinical Instrumentation - Nephelometry
7
Medical
Undergraduate 2
03/27/2012

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Term
Prinicipal
Definition
antigen/antibody complexes when formed at a high rate will precipiate out of solution resulting in a turbid our cloudy appearance
Term
Turbimimetry
Definition
measures the turbidity or cloudiness of a solution by measuring the amount of light directly passing through
Term
Nephelometry Indirect Measurement
Definition
measures amount of light scattered by the antigen/antibody comples
Term
Rayleigh Scattering
Definition
light scatters in every direction due to smaller particles
Term
Mie Scattering
Definition
light scatters mainly forward due to larger particles which cause a concentrated light scatter going forward
Term
Setup
Definition

light source --> sample --> unscattered light

 

scattered light

 

photocell

 

readout

Term
Disadvantages
Definition
must maintain a homogenous suspension
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