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Clinical Instrumentation - Flame Photometry
Clinical Instrumentation - Flame Photometry
6
Medical
Undergraduate 2
03/24/2012

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Term
Flame Photometry - Use
Definition

used to measure Na, K, Mg, and Li

 

good only for elements that are easily excited and don't require high temperatures

 

 

Term
Flame Photometry - Setup
Definition
flame --> monochrometer --> photodetector
Term
Flame Photometry - Fuel Types
Definition

acetylene

propane

oxygen

natural gas

compressed air

combo and type of fuel determine the temp, different metals require a different temp

Term
Flame Photometry - How It Works
Definition

1)sample matrix evaporates followed by atomization of the sample

2)atoms present in high temp zone are excited to higher levels by absorbing energy from the flame

3)as atoms return to the ground state radiation is emitted in a wavelength depending on the energy level the atoms dropped from

4)intensity of the color is proportional to amount of the element burned in the flame

Term
Flame Photometry - Monochromator
Definition
pre-selected filter (depending on the atom in question) is used and the intensity of the emission line is measure and is related to the concentration in the solution
Term
Flame Photometry - Photodetector
Definition
usually a phototube or photomultiplier
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