Term
| process of breaking open cells |
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| homogenization wont disrupt cell-cell communications such as |
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Definition
| desmosomes and tight junctions |
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| organelles r easier to seperate if the cells r slightly swollen |
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| use of a ___ buffer can b very beneficial n the isolation of mitochondria and mitotic chromosomes |
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| most common procedures use what 2 homogenizers |
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| addition of a motor driven teflon pestle creates the ___ homogenizer |
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Term
| process of rupturing a cell |
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| repeated high velocity compression and expansion is known as |
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| french press if favored for use when __ __ is required |
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| for cellular material what device is used |
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| forces a slurry of the cells thru an opening at very hi pressures |
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Term
| have been used to seperate organelles from cells, particulary from tissue culture cells |
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Definition
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Term
| once the cells have been homogenized the various components must be separated by the use of |
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| samples r allowed to sit and separation occurs due to the natural differences n size and shape (density) of the cells |
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Definition
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Term
| technique for fractionating cellular components is the use of |
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Definition
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Term
| procedures employing low speed instruments w/ greater volume capacity and refrigeration r known as |
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Definition
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Term
| particles n suspension can b separated by |
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Definition
| sedimentation velocity/equilibrium |
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Term
| sedimentation velocity (aka?) has the advantage of low speed centrifugation and short times, but yields incomplete separations |
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Definition
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Term
| sedimentation equilibrium (aka?) requires specimens to b subject to hi speeds 4 prolonged periods of time and seperates particles completely |
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Definition
| isopycnic or density equilibration |
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Term
| terminal velocity is dependent upon the relative centrifugal force (RCF) applied to the particle and is related to a math factor |
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Definition
| sedimentation coeffiecient/constant |
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Term
| sedimentation coefficient is determined by |
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Definition
| dividing the termianl velocity by centrifugal force field strength |
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Term
| diffusion coeffiecient (D) |
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Definition
| spread of molecule divided by time |
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Term
| sedimentation coefficient formula |
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Definition
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Term
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Definition
w= angular velocity of the rotor n rad/s 0.10472 x RPM
r= distance between the particle and center of rotation (mm)
dr/dt= rate of movement of the particle (cm/s) |
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Term
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Definition
| particles r centrifuged until they reach a density = to the media (equilibrium density separation |
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Term
| value for a molecule's sedimentation velocity n a centrifugal field is known as |
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Definition
| Svedberg constant (S value) |
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Term
| formula for time needed to completely sediment a particle can b determined by the formula |
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Definition
| T=((D-L)/(D+L)) * (N/(d(g-p)S)) |
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Term
| T=((D-L)/(D+L)) * (N/(d(g-p)S)) |
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Definition
T=time n min
D= RADIAL DISTANCE N CM FOR RMAX
L=RADIAL DIST TO MENISCUS
N=VISCOSITY OF THE FLUID MEDIUM
g=density of the fluid medium
p=density of the particle to sediment
d=diameter of the particle n cms
S=rotational velocity n RPM |
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Term
| working w/ the max. RCF for each roter gives the following conversion |
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Definition
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Definition
t1=run time needed for the Beckman rotor
ta=run time specified n the procedure
RCF1=RCF of JA-20 rotor @ max. speed
RCF2=RCF specified n the procedure |
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Term
| computing k(clearing factor) |
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Definition
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Term
| enzymes r studied __ __ after extraction from cells |
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Definition
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Term
| proteins can b divided nt albumins and globulins on the basis of their |
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Definition
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| as the salt concentration is increased, most proteins will |
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Definition
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Term
| the relationship between substrate concentration and enzyme concentration was math. establish by these 2 biochemist |
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Definition
| L.Michaelis and M.L.Menten |
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Term
| at __ all enzyme molecules r complexed w/ substrate and any additional substrate added to the reaction has no effect on the rate |
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Definition
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Term
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Definition
| concentration of substrate which wil giv exactly 1/2 vmax when reacted w/ an enzyme w/ max ph, temp, and cofactors |
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Term
| lineweaver/burke equation |
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Definition
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Term
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Definition
| enzyme units per mg enzyme protein |
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Definition
| amount of substrate converted to product per unit time under specific reaction conditions for pH and temp |
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Definition
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Term
| blood type A can give blood to and receive from |
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Definition
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Term
| blood type B can give/receive from |
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Definition
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Term
| blood type AB can give/receive from |
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Definition
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Term
| BLOOD TYPE O CAN GIV/RECEIVE FROM |
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Definition
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Term
| agglutination reaction of blood type A |
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Definition
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Term
| agglutination reaction of blood type B |
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Definition
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Term
| agglutination reaction of blood type AB |
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Definition
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Term
| agglutination reaction of blood type O |
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