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Biochem Lab Final UTSA
Biochem Lab Final UTSA test bank
19
Biology
Undergraduate 3
12/01/2012

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Term
Enzymes increase the rate of reaction by:
Definition
decreasing the energy of activation
Term
All of the following statements are true about Lysozyme enzyme except:
Definition
cleaves phosphate linkage between two carbohydrate moieties
Term
When [S]>> Km, which event can occur in enzyme catalyzed reaction?
Definition
The reaction is in zero order of reaction
Term
An apoenzyme is
Definition
an enzyme without cofactor
Term
What will be the initial velocity (vo) for an enzyme that has Km equal substrate concentration?
Definition
1/2 Vmax
Term
If Vmax =100 mM/sec and Km = 2 mM, what will be the initial velocity (vo) at [S] = 20 mM?
Definition
91 mM/sec
Term
A 0.1 ml pure Muramidase (0.5 mg/ml) hydrolyzes 0.2 mmol of substrate wall in 5 min. What is the specific activity?
Definition
800 mmol/min/mg
Term
Which of the following is the characteristic (s) of temperature curve in enzyme kinetic analysis?
Definition
bell shape
Term
A Lineweaver-Burk plot exhibit a slope of 5x10-4 M.min/mol and y-intercept of 0.1 min/mol. What will be the Km for this enzyme?
Definition
0.005 M
Term
Cholesterol can easily be metabolized to CO2 and H2O in humans and excreted.
Definition
F
Term
Which of the following binds plasma cholesterol and transport cholesterol to the liver?
Definition
HDL
Term
Most of your blood cholesterol is produced by:
Definition
Liver
Term
Cholesterol is synthesized from:
Definition
Acetyl CoA
Term
Which of the following is defined as the "turnover number for an enzyme?"
Definition
Kcat
Term
Cholesterol serves as a precursor for which of the following answer choice?
Definition
bile salts and steroid hormones
Term
Identify the competitive inhibitor for Acid phosphatase.
Definition
Sodium phosphate (Na2HPO4)
Term
Which of the following serves as a substrate for Acid phosphatase?
Definition
pNPP
Term
Tween-20 is a nonionic detergent and is used prior to colloidal gold reagent for total protein detection in western blot. The purpose of its use is to increase nonsepecific binding
Definition
F
Term
Western blot method is used to detect specific proteins by employing antibody against the protein of interest
Definition
T
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