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Bio Test 3
w00t
112
Biology
Undergraduate 2
10/21/2009

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Term
What is activation energy?
Definition
The initial amount of energy needed to start a chemical reaction.
Term
What is the transition state?
Definition
The summit of the reaction when the molecules are in an unstable state.
Term
How do enzymes accelerate reactions and lower activation energy requirements?
Definition
They provide an environment for the chemical reaction to proceed.
Term
What is a cofactor? A coenzyme?
Definition
A nonprotein enzyme helper. Coenzymes are organic cofactors for enzymes.
Term
What is a competitive inhibitor?
Definition
An imitator of a substrate that binds to the active site of an enzyme, competing with the substrate.
Term
What is a noncompetitive inhibitor?
Definition
An inhibitor that binds to another part of an enzyme that changes the function.
Term
What is a distinguishing characteristic of living things from nonliving matter?
Definition
The ability of organisms to reproduce.
Term
What is a genome?
Definition
A cell's endowment of DNA, genetic information.
Term
What is chromatin?
Definition
A complex of DNA and protein that condenses during cell division.
Term
Where are chromatids connected?
Definition
Centromeres.
Term
What happens in meiosis?
Definition
Sex cells have a reduction in chromosome number, yield 4 nonidentical daughter cells, and each with half the chromosomes of the parent.
Term
What occurs in prophase?
Definition
The chromosomes are tighttly packed, chromatids are joined, and mitotic spindle begins to form.
Term
What occurs in prometaphase?
Definition
The nuclear envelope fragments, the microtubules from the spindle interact with the chromosomes and the chromatids contain a kinetochore.
Term
What occurs in metaphase?
Definition
Sister chromatids are arranged at the metaphase plate.
Term
What occurs in anaphase?
Definition
The centromeres divide separating the sister chromatids which are pulled toward the poles.
Term
What occurs int telophase?
Definition
Daughter nuclei begin to form at the 2 poles and nuclear envelopes begin to arise.
Term
What facilitates cytokinesis?
Definition
Actin microfilaments that form a contractile ring.
Term
What type of cell division do bacteria use?
Definition
Binary fission- the bacterial chromosome, circular DNA, repicates and the two daughter chromosomes separate.
Term
What regulate the progress through the cell cycle?
Definition
Molecules and enzymes in the cytoplasm.
Term
How many major chechpoints are there in the cell cycle? Which is typically the most important?
Definition
There are three (G1, G2, M). G1 is typically the most important.
Term
What are catabolic pathways?
Definition
They break down complex molecules into simpler compounds during a process that releases energy.
Term
What do anabolic pathways do?
Definition
They build complicated molecules from simpler ones during a process that consumes energy.
Term
Define thermodynamics.
Definition
The study of energy transformations and life is subject to the laws of thermodynamics.
Term
What is the first law of thermodynamics?
Definition
Energy can be transferred and transformed but cannot be created or destroyed.
Term
What is entropy?
Definition
The quantity of disorder or randomness. The more random, the greater the entropy.
Term
What is the second law of thermodynamics?
Definition
Every energy transfer or transformation increases the entropy of the universe. Order may increase locally, however the universe tends ttoward randomness.
Term
What is free energy?
Definition
Energy that can do work under cellular conditions (constant temperature and pressure).
Term
What dictates spontaneity of a reaction? Why is this important?
Definition
Free energy change of a reaction dictates spontaneity. Spontaneous changes do not require energy which is preferred for cellular reactions.
Term
What is the formula for the change in free energy?
Definition
Change in free energy = enthalpy change - T(change in entropy).
Term
What is the importance of free energy change?
Definition
When free energy decreases, stability of a system increases. When free energy change is negative, the more work a spontaneous process can perform.
Term
Are exergonic reactions spontaneous?
Definition
Yes, exergonic reactions are spontaneous, and endergonic are not spantaneous (positive free energy change).
Term
Do cells maintain a system of equilibrium?
Definition
No, cells tend toward disequilibrium because they are open system.
Term
List and define the three kinds of work that cells do.
Definition
Mechanical: the beating of cilia/flagella or contracting muscles. Transport: pumping substances across membranes. Chemical: Driving endergonic reactions such as synthesis of polymers from monomers.
Term
When is energy released from ATP?
Definition
When the terminal phosphate bond is broken.
Term
How do cells make reactions favorable?
Definition
By coupling ATP hydrolysis with other reactions.
Term
How do enzymes regulate reactions in metabolic pathways?
Definition
By bond breaking and forming.
Term
What does sucrase do?
Definition
Catalyzes the hydrolysis of sucrose (breaking of the bond between glucose and fructose).
Term
What is allosteric regulation?
Definition
Protein's function at one site is affected by binding of a regulatory molecule at another site.
Term
What do allosteric enzymes do?
Definition
Change shape when regulatory molecules bind to specific site affecting function.
Term
What is cooperativity?
Definition
A form of allosteric regulation that can amplify enzyme activity.
Term
What is feedback inhibition? Why is this important?
Definition
The end product of a metabolic pathway feeds back upon itself and shuts down the pathway. It prevents a cell from wasting chemical energy.
Term
How does energy enter and exit most ecosystems?
Definition
Enters as sunlight, leaves as heat.
Term
What is fermentation?
Definition
Partial degradation of sugars that occurs in the absence of oxygen.
Term
What dictates catabolic pathways' ability to yield energy?
Definition
The ease in which they transfer electrons.
Term
What are redox reactions?
Definition
The transfer of electrons (energy) from one reactant to another by oxidation and reduction.
Term
What happens in oxidation?
Definition
A substance loses electrons (is oxidized).
Term
What happens in reduction?
Definition
A substances gains electrons (is reduced).
Term
What is an example of a redox reaction?
Definition
The formation of table salt. Sodium oxidized, chlorine reduced.
Term
What is being oxidized and reduced in cellular respiration?
Definition
Glucose is oxidized. Oxygen is reducd.
Term
What does dehydrogenase do?
Definition
Removes 2 hydrogen atoms from glucose and transfers 2 electrons and one proton to Nad+ during cellular respiration.
Term
Where is the electron transport chain located?
Definition
Mitochondria.
Term
How does the electron transport chain "break the fall" of electrons? What does it do with the energy resulting from this process?
Definition
By passing them in a controlled series of steps. The energy from electron transfer is used make ATP.
Term
What occurs at the lower end of the electron transport chain?
Definition
Oxygen captures the electrons along with hydrogen to form H2O.
Term
What is respiration a cumulative function of?
Definition
Glycolysis, CAC, and oxy.phos.
Term
Where do each of the three parts of respiration occur?
Definition
Glycolysis occurs in the cytoplasm, while Krebs and Oxy take place in the mitochondria.
Term
Which parts of respiration generate ATP by substrate-level phosphorylation?
Definition
Glycolysis and CAC. Oxyphos is oxidatize phosphorylation.
Term
What occurs in substrate-level phosphorylation?
Definition
An enzyme transfers a phosphate group from an organic structure to ADP, forming ATP.
Term
What does hexokinase do?
Definition
Phosphorylates glucose.
Term
What two phases are a part of glycolysis?
Definition
Energy investment phase and the energy payoff phase.
Term
What occurs between glycolysis and CAC?
Definition
Pyruvate is transported and cnverted to acetyl CoA.
Term
What does the CAC produce per molecule of glucose?
Definition
2FADH, 6NADH, 2ATP, 4CO2.
Term
What does the transport between glycolysis and CAC yield per molecule of glucose?
Definition
2CO2, 2NADH, 2Aceytl CoA
Term
What accounts for the majority of energy extracted from food?
Definition
NADH and FADH
Term
What are NADH and FADH's functions with respect to the electron transport chain? Why is this significant?
Definition
They donate electrons. This powers ATP synthesis via oxidative phosphorylation.
Term
Do NADH and FADH lose or gain energy in the electron transport chain?
Definition
They lose energy until the electrons are passed to oxygen, forming water.
Term
What is ATP synthase?
Definition
ATP synthase is a large protein complex imbedded in the mitochondrial wall and is the enzyme that synthesizes ATP.
Term
What is the purpose of the H+ gradient created in oxidative phosphorylation?
Definition
It stores energy and drives chemiosmosis (through ATP synthase).
Term
What is chemiosmosis?
Definition
An energy-coupling mechanism that uses energy in the form of a H+ gradient across a membrane to drive cellular work and the formation of ATP.
Term
What sequence does most energy flow in during respiration?
Definition
Glucose to NADH to electron transport chain to proton-motive force to ATP.
Term
What is the yield of one molecule of glucose during glycolysis and the CAC?
Definition
6 CO2, 4 ATP.
Term
How much ATP is generated from glucose and what is the breakdown?
Definition
34 from oxyphos, 4 from substrate level. 36-38 overall.
Term
What percent of energy in a glucose molecule is transferred to ATP during cellular respiration?
Definition
40%
Term
What is the importance of electronegative oxygen?
Definition
Without is, oxyphos stops.
Term
What does fermentation do?
Definition
It enables some cells to produce ATP without use of oxygen. It can generate ATP from glucose by substrate-level phosphorylation as long as there is a supply of NAD+ to accept electrons.
Term
What is pyruvate converted to in alcohol and lactic acid fermentation?
Definition
In alcohol fermentation, it is converted to ehtanol in two steps, releasing CO2 in one of them. In lactic acid fermentation, pyruvate is reduced directly to NADH to form lactate as a waste product.
Term
What is the difference between fermentation and cellular respiration? What is the same?
Definition
They both use glycolysis to oxidize glucose and other organic fuels to pyruvate but respiration produces more ATP.
Term
What controls cellular respiration?
Definition
Allosteric enzymes.
Term
What is photosynthesis?
Definition
The process that converts solar energy into chemical energy and helps nourish the biosphere.
Term
What do plant chloroplasts use photosynthesis for?
Definition
Capturing light energy from the sun and converting it into stored sugars and organic molecules.
Term
What are the two types of autotrophs?
Definition
Photoautotrophs or chemoauthotrophs?
Term
What type of autotroph are plants?
Definition
Plants are photoautotrophs because they use the energy of sunlight to make organic molecules from water and CO2.
Term
What are the major sites of photosynthesis? What does leaf color come from?
Definition
Leaves are the major site of photosynthesis. The color comes fro the chlorophyll, a green pigments in the chloroplasts.
Term
What are stomata?
Definition
Stomata are the pores in the leaf through which O2 and CO2 leave and enter.
Term
What are chloroplasts?
Definition
The organelles in which photosynthesis occurs
Term
What is the stroma?
Definition
The aqueous space in chloroplasts.
Term
Where are thylakoids and grana contained?
Definition
Within the stroma.
Term
What type of reaction is photosynthesis?
Definition
Anabolic.
Term
Is photosynthesis a redox process?
Definition
Yes, water is oxidized and CO2 is reduced.
Term
What are photo reactions that occur in photosynthesis?
Definition
Occurring in the grana, they split water, release oxygen, produce ATP, and form NADPH.
Term
What is the Calvin Cycle?
Definition
It occurs in the stroma, forms sugar from Carbon dioxide, uses ATP for energy, and NADPH for reducing power.
Term
What form of chemical energy do light reactions convert solar energy to?
Definition
ATP and NADPH.
Term
What is a pigment? Give an example.
Definition
Substances that absorb visible light and reflect the light that we see. An example is chlorophyl A.
Term
What is an action spectrum of pigment?
Definition
It profiles the relative effectiveness of different wavelengths of radiation in driving photosynthesis.
Term
What happens when a pigment absorbs light?
Definition
Its electrons become unstable and it goes from a ground state to an excited state that releases heat energy and sometimes fluorescence.
Term
What are chlorophyll and other proteins organized into?
Definition
Photosystems.
Term
What do light-harvesting complexes do?
Definition
They funnel energy of photons of light to the reaction center.
Term
What does the reaction-center chlorophyll do?
Definition
They absorb energy and as the electrons "fall" to a lower energy level, their energy is harnessed to produce ATP.
Term
Do chloroplasts or mitochondria use the H+ gradient?
Definition
Both use it. Both use ATP synthase and proton motive force to make ATP.
Term
What does the Calvin Cycle do?
Definition
ATP and NADPH to convert CO2 to sugar.
Term
Where does the Calvin Cyle occur? What three phases does it have?
Definition
It occurs in the stroma. The three phases are Carbon fixation, reduction, regeneration of the CO2 acceptor.
Term
What does one turn of the CAC yield?
Definition
3NADH, 1FADH
Term
Two molecules of glucose enter cellular respiration. At the end of CAC, how much ATP, NADH, FADH will be made?
Definition
8 ATP, 20 NADH, 4 FADH
Term
What is the significance of the NADH/FADH produced in the CAC?
Definition
It is taken to the electron transport chain, and used to eventually make ATP via ATP synthase.
Term
What is an allosteric activator? An allosteric inhibitor?
Definition
Activator binds to an enzyme and increases the speed of reaction, inhibitor stops the function. Example of an activator is glucose for hexokinase. Example of an inhibitor is ATP for hexokinase.
Term
What are the two parts of oxidative phosphorylation?
Definition
Electron transport chain and chemiosmosis.
Term
What is produced via a light reaction?
Definition
ATP, NADPH, O2
Term
What occurs in the Calvin Cycle?
Definition
It takes in ATP and NADPH and produces sugar, ADP, and NADP.
Term
What is the difference between catabolic and anabolic?
Definition
Catabolic (such as cellular respiration) makes ATP as a result. Anabolic takes ATP and makes something from it (such as photosynthesis).
Term
Are living systems closed?
Definition
No, living systems are open because they interact with the things around them, expending and accepting energy.
Term
What does dehydrogenase do?
Definition
Helps make NADH by adding the H.
Term
Where does the Calvin Cycle occur?
Definition
Stroma.
Term
Where do light reactions of photosynthesis occur?
Definition
Thylakoid.
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