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Bio 1015 Snow College Gordillo
Lab Final
69
Biology
Undergraduate 3
05/23/2011

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Term
Why did DNA and RNA give a + MOLISH TEST?
Definition
they have sugar in them
Term
The NINHYDRIN test tested for what group of compounds?
Definition
Amino Acids
Term
The translucency test tested for what?
Definition
Lipids
Term
The functional group of carbohydrates?
Definition
Sugars
Term
The Functional group of Nucleic Acids are?
Definition
Nucleotides
Term
Occular Lens (on microscope)
Definition
eye piece lens
Term
Course and Fine Focus (on microscope)
Definition
knobs on either side that have a small one outside of the larger one
Term
Stage (on microscope)
Definition
What you clip the slide on
Term
Which lens (on microscope) should never be used in this class?
Definition
Oil Immersion (100X)
Term
When you look at an "e" under the microscope it appears _______ and _______
Definition
upsidedown and backwards
Term
The parts of a cell that can be seen under a light microscope
Definition
Nucleus
Plasma Membrane
Cell Wall (if present)
Chloroplasts (if present)
Term
Milk has a pH of 6.6 so it is _________
Definition
Acidic
Term
The enzyme catalase was active in ________ and ________ situations
Definition
basic and nuetral
(a low pH denatures catalase)
Term
The distilled water was ________ in relationship with the onion
Definition
HYPOtonic
Term
The 0.9% salt soln was ________ to the onion
Definition
ISOtonic
Term
The 5% salt soln was _________ to the onion
Definition
HYPERtonic
Term
What part of the visible light spectrum is MOST efficient in running photosynthesis?
Definition
White is most eff, then Blue is next then Red
Term
What compound is made during BOTH photophosphorylation and oxidative phosphorylation?
Definition
ATP
Term
Increasing the CO2 concentration increases the rate of___________
Definition
the light independent reaction
Term
When the light was moved closer to the plant it caused an increase in the rate of ___________
Definition
the light dependent rxn
Term
The citric acid cycle occurs in _________
Definition
respiration
Term
A normal cell in TELOPHASE 1 of Meiosis has __N chromosomes?
Definition
2
Term
What is occurring in a cell during Metaphase?
Definition
Chromosomes line up in the center of cell
Term
What is occurring in a cell during PROPHASE 2 of Meiosis?
Definition
the chromosomes become visible and microtubules lengthen
Term
If a cell normally has 44 chromosomes, how many would be present at PROPHASE 2 of Meiosis?
Definition
22
Term
Replication of DNA occurs during ________
Definition
Interphase
Term
Megasporogenesis
Definition
In plants it is the development of the female gamete (ovule)
Term
Microsporogenesis
Definition
In plants, it is the development of the male gamete (pollen grain)
Term
In the semeniferous tubule of the testis, spermatozoa move from the surrounding cells into the center of the tubule called the _________
Definition
Lumen
Term
Define Sporophyte
Definition
A plant that is 2N that matures to produce gametes- Micro and Megaspores
Term
Define Double Fertilization
Definition
In plants, after the ovule (megaspore) has undergone mitosis and grown into an 8 nuclear cell, the pollen comes in with 2 sperm cells and 1 fertilizes the 2 connected polar bodies (makes a 3N endosperm) and the other fertilizes the ovule (2N). This all becomes the embryo sac.
Term
What is a test cross?
Definition
Cross your unknown with a double recessive (Aa X aa)
Term
When counting the corn my table obtained the following data for the F2 cross:; 45 Yel. Shrunk, 144 Yel. Full, 147 Pur. Shrunk, 392 Pur. Full- When we resolved the data we should have had the following ratio:
Definition
9:3:3:1
Term
When you add 2 monohybrid crosses (of 2 different traits) together, you get a ________ cross.
Definition
Dihybrid
Term
The possible gametes for an individual with a genotype of SSRr are:
Definition
SR, Sr, SR, Sr
Term
F2 generation refers to what?
Definition
Grandchildren
Term
What is a plasmid?
Definition
Circular piece of DNA
Term
What is the PUC18 we used last week in our lab?
Definition
plasmid is a circular dDNA molecule  that are usually viruses of bacteria when found naturally. Artificial plasmid pUC18 has been genetically engineered to include a gene for antibiotic resistance to Ampicillin (ampR), and a  gene for the enzyme beta-galactosidase (lacZ).
Term
2 markers on PUC18?
Definition
Amp-R (ampicillin resistance marker)
LACz (Lactose marker)
Term
What is the medium used in the petri dish
Definition
Nutrient agar
Term
What is/was c-1
Definition
control #1 (no plasmid added)
Term
What's P-1
Definition
Only plasmid added (e coli added to see results)
Term
What was C - A
Definition
Control with ampicillin
Term
What was P A X
Definition
Plasmid one that we added ecoli and Ampicillin and the lactose (substitute)
Term
Using DNA gel electrophoresis, DNA fragments move toward_____ electrode?
Definition
Positive
Term
DNA electrophoresis- the fragments move to a distance that is inversely proportional to the DNA fragments ______?
Definition
Size
Term
How can you tell which DNA matches?
Definition
lines appear on same mm line on both samples
Term
Diagnosing DNA match, is 1 matching line enough to prove someone guilty?
Definition
No
Term
The 3 domains in life?
Definition
Bacteria
Archaea
Eukarya
Term
5 Kingdoms
Definition
Chromista
Protista
Plantae
Fungi
Anamalia
Term
Shapes of Bacteria
Definition
Coccus, Spirillium (Spirochete), Bacillus
Term
What is the difference between Gram + and Gram -?
Definition
Gm+ stains purple b/c of a THICK peptidoglycan layer
GM- stains pink- THIN
Term
2 types of microorganisms grow on your petri dish, what are they and what do they look like?
Definition
1) Bacteria- looks creamy colored pink/red
2) Fungi- cottony or fuzzy looking, brownish or blackish
Term
Mushrooms belong to
Definition
Basidiomycota division
Term
Fungi in Lichens belong to
Definition
Acomycota division
Term
Black bread mold belongs to
Definition
Zygomycota division
Term
The protistian phylum that is responsible for MALARIA is
Definition
Apicomplexa
Term
The protistan phylum which has cilia is
Definition
Ciliophora
Term
Plants that have fronds and sori belong to
Definition
Pterophyta division
Term
Plants that have no vascular tissue and are haploid dominant belong to
Definition
Bryophyta division
Term
Plants that have flowers belong to
Definition
Anthophyta division
Term
Monocots have _____ cotelydon(s) and _______ and __________ and ________
Definition
MONO= 1
Parallel leaf veination
Flower Parts in multiples of 3
Fibrous Rood
Term
Dicots have
Definition
2 cotelydon
Webbed leaf veination
Vascular bundles in rings
Flower parts in multiples of 4 or 5
Taproot
Term
What is the most visible distinguishing characteristic of MAMMALS
Definition
Hair
Term
What phylum and class do lizzards belong to?
Definition
Chordata, Reptile
Term
Sea stars belong to the class
Definition
Astrodia
Term
Common name for organisms in the class ANTHOZOA (which is within the Phylum Cnidarian)
Definition
Coral
Term
Birds belong to phylum
Definition
Chordata
Term
DNA and RNA base pairing
Definition
C--G
A--T (but in RNA there's no T so A--U)
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