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8/24 Histology - Cyto Organs
Maz Theme 1
17
Biology
Graduate
08/29/2011

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Term
Fixing Tissue entails...
Definition
- Removing water in the sample and replacing with soluble solvent with embedding medium
- A harder medium allows the tissue to be sliced thinly
Term
Light Microscopy
Definition
- Resolution of .2 micrometers
- Fixed in aqueous formaldehyde (proteins and NucAcids)
- Sample is dehydrated thru 50-70-90-100 EtOH washes
- Embedded in paraffin wax
- Slice 5-15 micrometers
Term
Electron Microscopy
Definition
- Fixed in glutaraldehyde then ozmium tetroxide
- Theoretical resolution .05nm, 1nm in actual bio sample
- Dehydratd like LM (using progressive EtOH)
- Embed in epoxy
- Plastics allow 50-150 nm (.05-15um)
Term
Angstrom
Definition
- 1A = .1nm
- 10 A = 1 nm
Term
Hematoxylin
Definition
- Basic dye, + charge
- Stains PO4 gprs of NAs, sulfates of glycoaminoglycans, carboxyl groups of proteins; basophiles
- Dark purple
Term
Eosin
Definition
- Acid dye; - charge
- Binds to cationic components
- Light purple
Term
PAS
Definition
- stains carbs; gluco-proteins/lipids/gens
- saints extracell comp like t3 collagen and glycosaminoglycans
Term
Toluidine blue
Definition
- Metachromatic; colors diff comp diff
- Basic dye
- More bound goes from blue to purple
- ex; Heparin granules of mast cells
Term
Flourescence Microscopy
Definition
- Raise antibody to an antigen
- Binds to that antigen
- Secondary antibody recognize primary anti-body and ave marker that allow detection
- Common markers; dyes (LM), HRP (L+E/M), gold spheres (EM), alk phosphotase/peroxidase for biochem
Term
DAPI
Definition
- 4', 6-diamidino-2 phenylindole; fluorsces blue when bound to DNA
Term
GFP-PKC
Definition
- Flourescent tag
- Causes translation of tagged PKC
- Allows to track localization of PKC in cell
Term
Opsonized Red beads
Definition
- Beads that flouresce red
- Allows to track phagocytosis
- Ex; macrophage
Term
Uranium
Definition
- EM stain
Term
Gold-labeled anti-bodies
Definition
- http://www.topatoco.com/merchant.mvc?Screen=PROD&Store_Code=TO&Product_Code=NED-MAGIC&Category_Code=NED
Term
Membrane resolution
Definition
- EM can LM can't
Term
Terminal bar
Definition
- Junction sites between adjacent cells, darker spots between cells along the surface
- At EM levels it is a complex
Term
Microtubules
Definition
Green usually (outer most)
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